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Construction of a transcriptional-translational regulation platform using a novel poly(A) determination method.

Construction of a transcriptional-translational regulation platform using a novel poly(A) determination method.
使用新型聚腺苷酸测定方法构建转录翻译调控平台。
批准号:
24651212
负责人:
TEI Hajime
金额:
$2.58万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2013-03-31

项目摘要

项目成果

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中文摘要
翻译
我构建了一种检测真核生物mRNA中poly(a)的新方法,命名为PACHINCO RT-PCR。该方法对自动芯片DNA测序仪的应用进行了优化,并明确了Per1时钟基因转录本的poly(A)延伸率。随后,将该方法应用于NGS测序仪,提高了poly(A)测定的通量和分析精度。因此,我在大鼠视交叉上核衍生细胞系和小鼠视交叉上细胞中收集了具有poly(A)长度昼夜振荡的mrna。
英文摘要
I have constructed a novel method for the determination of poly(A) in eukaryotic mRNA, and designated as PACHINCO RT-PCR. The method was optimized for the application of automatic microchip DNA sequencers, and the elongation of the poly(A) of Per1 clock gene transcripts was clearly determined. Subsequently, the method was applied for NGS sequencers for the improvement of the throughput and analytical precision for poly(A) determination. Accordingly, I have collected mRNAs with the circadian oscillation of poly(A) length in both a rat suprachiasmatic nucleus derived cell line and mouse suprachiasmatic cells.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
時計遺伝子、転写・翻訳フィードバックループと概日リズム
时钟基因、转录/翻译反馈环和昼夜节律
DOI: --
发表时间: 2013
期刊:
影响因子: --
作者: [Eriko Kakegawa, Yasuyuki Shimizu, Ichiro Kimura, Yoshihiko Saito and Evgeny Isenko, イエリン賢太郎・斎藤佳彦・木村一郎・大槻政哉・イセンコエフゲニー・清水康行, イエリン賢太郎・斎藤佳彦・木村一郎・大槻政哉・イセンコエフゲニー・清水康行, 程 肇]
通讯作者: 程 肇
Development of translational regulation platform of circadian rhythms using a novel poly(A) determination method
  • 批准号:
    25640100
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.58万
  • 财政年份:
    2013
  • 负责人:
    TEI Hajime
  • 依托单位:
A Genome-wide screening of circadian transcripts using a novel poly(A) determination method
  • 批准号:
    23651184
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.58万
  • 财政年份:
    2011
  • 负责人:
    TEI Hajime
  • 依托单位:
Metabolomics Analysis of Phase Shift and Intercellular Entrainment of Central Circadian Pacemaker Cells
Screening of Molecules for entrainment of circadian rhythms in mammalian central and peripheral clock cells
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