Development of polymeric macroporous hydrogels for the immobilization of enzymes using an emulsion-gelation method

为改进酶反应工艺，研制了新型高分子大孔水凝胶。采用乳化-凝胶法制备凝胶，将酶包埋在随机分布的非连通球形大孔中。将脂肪酶固定在大孔水凝胶和有机凝胶中，分别成功催化了水介质中的水解和有机介质中的酯交换反应。

Novel polymeric macroporous hydrogels were developed to entrap and immobilize enzymes for improved enzyme reaction process. The emulsion-gelation method using emulsions was used to simultaneously synthesize the gels and entrap enzymes in the randomly distributed, non-interconnected, sphere-like macropores. The lipase, immobilized within the macroporous hydrogel and organogel, successfully catalyzed the hydrolysis in aqueous media and the transesterification in organic media, respectively.