Neural mechanism of ultrasonic vocalization of mice

小鼠的超声发声是由一系列发声要素组成的。因此，超声发声的神经机制备受关注。在本研究中，我们根据听力损失来研究DBA2/J小鼠的USV的变化。结果表明，发声成分的平均基频在6~8周龄时随发育效应而降低，而在11~13周时随听力损失而升高。我们还对包括听觉皮层在内的SAM小鼠急性脑片进行了-ch多点记录的体外实验。利用电流源密度分析，我们可视化了听觉皮质中的时空动力学，即从深层到浅层的初始活动传播，以及沿水平方向的附加传播。

Ultrasonic vocalization (USV) of mice is composed of a sequence of vocal elements. Therefore, neural mechanism of ultrasonic vocalization has intensely been focused. In this study, we investigated changes in USVs of DBA2/J mice according to hearing loss. As a result, the average fundamental frequency of vocal elements was decreased in 6 ~ 8 weeks of mice as developmental effect, while it was increased in 11 ~ 13 weeks of mice according to hearing loss. We also executed in vitro experiments for 64-ch multi-site recording from acute brain slices including auditory cortex of SAM mice. Using current source density analysis, we visualized spatiotemporal dynamics in the auditory cortex, which are initial activity propagations from deep to shallow layer, and additional propagations along horizontal direction.