Experimental development a new cryo-holder suitable to electron cryo-tomography

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The new cryo-holder suited to electron cryo-tomography was planed by using low-vibration cryo-pump and has been developed. Furthermore, conventional liquid-nitrogen-type cryo-holder was tested and successfully achieved a lower-temperature stage than conventional one by drawing vacuum with vacuum pumps. Hoses for vacuum drawing were important for less vibration of the stage and better hoses give the stable stage to obtain electro micrographs. Currently we are planning to develop a new holder by combining both techniques.