Dynamic conformational changes that regulate the energy-converting unit

PomA/PomB复合体在细菌鞭毛马达中起着能量转换定子单元的作用，当安装到转子周围的适当位置时，它就会发挥作用。在功能性定子中，据信诱导PomB的周质区域中的构象变化以将其锚并同时激活定子的离子传导活性。本研究的目的是检测这样的构象变化，使用荧光探针和纯化的PomA/B复合物重组到脂质体中。我们还在建立分析体系的过程中，但我们大大改进了PomA/B复合物的过量生产和纯化程序，以便我们可以系统地筛选将功能定子复合物重组到脂质体的条件。我们还成功地缩小了构象变化发生的区域，通过使用系统的二硫键形成分析。

The PomA/PomB complex, that functions as an energy-converting stator unit in the bacterial flagellar motor, becomes functional when installed into the appropriate place around the rotor. In the functional stator, it is believed that a conformational change in the periplasmic region of PomB is induced to anchor it and simultaneously to activate ion conduction activity of the stator. This study aims to detect such a conformational change using the fluorescent probe and the purified PomA/B complex reconstituted into the liposome. We are still on the way to establish the analyzing system, but we greatly improved the overproduction and purification procedure for the PomA/B complex, so that we could systematically screen conditions to reconstitute the functional stator complex to the liposome. We also succeeded in narrowing down the region where the conformational change occurs, by using the systematic disulfide bridge formation analysis.