Development of novel method for searching plant disease resistance gene based on the pathogen perception mechanism

我们测试了是否有可能基于与已知抗性蛋白相互作用的已知蛋白因子的相互作用分离新的植物抗病基因。从茄子品种中筛选辣椒多巴病毒抗性基因L的部分同源cDNA序列，并与辣椒L抗性蛋白的互作伙伴LIPM1和LIPM2进行互作。结果表明，LIPM1和LIPM2与茄子中多种l -同源序列相互作用，不太可能参与抗性蛋白病原菌特异性的测定。基因沉默研究提示LIPM2参与细胞死亡信号的调控。

We tested whether or not it is possible to isolate novel plant disease resistance gene based on the interaction with protein factors known to interact with known resistance protein. Partial cDNA sequences homologous to pepper tobamovirus resistance gene, L, from an eggplant cultivar were screened for the interaction with LIPM1 and LIPM2, both of which were identified as interaction partners of pepper L resistance protein. The results suggest that the both LIPM1 and LIPM2 interact with diverse L-homolog sequences from eggplant and that they are unlikely to be involved in the determination of pathogen specificity of resistance protein. Gene-silencing study suggest that LIPM2 has a role in the regulation of cell-death signaling.