Functional analysis of hyphal sheath of white-rot fungus for lignin conversion bioreactor construction

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White-rot fungus was cultivated in ligninolytic conditions and was histochemically analyzed distribution of lignin-degrading enzyme activity and hyphal sheath. Lignin-degrading enzyme reaction was observed as peroxidase activity staining at hyphal tips after 2-3 days cultivation and was observed to extended in hyphal sheath after 3-4 days of cultivation. It was confirmed that the enzyme, which produced peroxidase activity staining, is manganese peroxidase. N-terminal amino acid sequence of the enzyme protein was consistent with manganese peroxidase gene sequences. These results showed that the manganese peroxidase reaction occurred in hyphal sheath of a white rot fungus, and suggested that hyphal sheath of white-rot fungus functioned as a lignin-degrading bioreactor.