Selective isolation of pseudopodia by using excimer laser ablation

伪足（富含肌动蛋白的突起）被认为是癌症转移的重要因素。将肿瘤细胞培养在多孔膜上以在膜孔中形成伪足。采用准分子激光去除膜上的细胞体，选择性分离膜孔中的伪足。对伪足中提取的蛋白质进行了分析鉴定。在这些蛋白质中，α-Parvin，其在伪足中高度表达，被表征。结果表明，该蛋白与伪足的长度、密度及细胞迁移能力有关。

Pseudopodia (actin-rich protrusion) are considered to be important for cancer metastasis. Tumor cells were cultured on a porous membrane to form pseudopodia in membrane pores. An excimer laser, which was used for LASIK, was employed for removing cell body on a membrane to selectively isolate pseudopodia in membrane pores. The proteins extracted from pseudopodia were analyzed and identified. Of those proteins, alpha-Parvin, which highly expressed in pseudopodia, was characterized. The results showed that the protein was involved in pseudopodial length and density, and cell migration ability.