Establishment of new extraction system from bone marrow smear by laser microdissection

我们建立了一种新的激光显微切割系统，从短暂性异常骨髓细胞生成（TAM）或唐氏综合征急性髓系白血病（AML-DS）骨髓涂片中提取白血病原始细胞的cDNA和mRNA。我们在TAM患者的骨髓标本和显微解剖系统中检测到相同的GATA 1突变。我们还可以通过这种显微切割系统从mRNA中检测GAPDH，并通过RT-PCR测量CXCR 4的表达。我们将能够通过这种显微切割系统通过下一代测序从AML或MDS患者中检测新的预后因素。

We established a new laser microdissection system extracting cDNA and mRNA of leukemic blasts from smear of bone marrow samples of transient abnormal myelopoiesis (TAM) or acute myeloid leukemia with Down syndrome (AML-DS). We could detect same GATA1 mutation in the TAM patient both from bone marrow samples and from the microdissecton system. We could also detect GAPDH from mRNA through this microdissection system and measured CXCR4 expression by RT-PCR. We will be able to detect new prognostic factors from AML or MDS patients by next generation sequencing through this microdissection system.