Role of fatty acid elongase Elovl6 in the sucrose preference formation

我们研究了Elov16缺乏对小鼠摄食和食物偏好的影响。在本研究中使用三种类型的饮食，其中一种或两种饮食同时可用于野生型（WT）和Elov16敲除（KO）小鼠。在实验1中，给小鼠喂食标准食物、高脂肪或高蔗糖饮食。在实验2中，给WT和Elov16KO小鼠喂食三对饮食中的一对，食物和HF、HS和HF或食物和HS。在单独的笼内小鼠中顺序监测食物摄入。在实验1中，Elov16KO小鼠显示出比WT小鼠增强的HS饮食摄取。相比之下，WT和Elov16KO小鼠之间的标准食物和HF饮食摄入相似。在实验2中，与WT小鼠相比，Elov16KO小鼠显示出对HS饮食比普通食物饮食更大的偏好，但表现出对普通食物相对于HF饮食或HS相对于HF饮食的同等偏好。对于食物和HS饮食，WT和Elov16 KO小鼠之间的食物和卡路里摄入总量（HS和食物饮食的总和）相似，因为Elov16 KO小鼠中食物饮食消耗在所有时间点都减少。Elov16KO小鼠表现出对HS饮食的明显增强的偏好。这些结果表明Elov16可能在控制享乐性进食如蔗糖适口性中发挥特定和独特的作用，但对稳态进食没有影响。

We investigated the effects of Elovl6 deficiency on the food intake and food preference in mice. Three types of diets were used in this study, with one or two diets simultaneously available to wild-type (WT) and Elovl6 knockout (KO) mice. In Experiment 1, mice were fed a standard chow, high fat, or high sucrose diet. In Experiment 2, WT and Elovl6KO mice were fed one of three pairs of diet, chow and HF, HS and HF, or chow and HS. Food intake was sequentially monitored in individually caged mice. In Experiment 1, Elovl6KO mice showed an enhanced ingestion of HS diet than WT mice. In contrast, the standard chow and HF diet intake were similar between WT and Elovl6KO mice. In Experiment 2, as compared with WT mice, Elovl6KO mice showed a greater preference for HS diet than a chow diet, but exhibited an equivalent preference for chow versus HF diet, or HS versus HF diet. With chow and HS diets, the total amount of food and calorie intake, which are the sum of HS and chow diet, were similar between WT and Elovl6 KO mice because chow diet consumption was decreased at all time point in Elovl6KO mice. Elovl6KO mice demonstrated a clear enhanced preference for HS diet. These results indicate that Elovl6 may play a specific and unique role in the control of hedonic eating such as sucrose palatability, but have no effect on homeostatic eating.