Lipid transport across membrane contact sites (25*)

细胞膜接触位点是细胞器之间进行信号转导和物质交换的平台。一些证据指向脂质在这些接触中的协调转运，但由于技术障碍，目前难以直接观察完整细胞中的脂质转运。在这个项目中，我们将开发基于三功能脂质原理的新型探针，这些探针能够预定位，释放和可视化以及识别脂质相互作用蛋白。此外，我们计划采用化学诱导的，细胞器之间的合成系链，以调查膜接触部位的病理，如溶酶体贮积症和丙型肝炎病毒复制的贡献。

Membrane contact sites serve as platforms for signal transduction and exchange of material between organelles. Several lines of evidence point towards coordinated transport of lipids across such contacts, but it is currently difficult to directly visualize lipid transport in intact cells due to technical obstacles. In this project, we will develop novel probes based on the trifunctional lipid principle which enable pre-localization, release and visualization as well as identification of lipid-interacting proteins. Additionally, we plan to employ chemically inducible, synthetic tethers between organelles to investigate the contribution of membrane contact sites to pathologies such as lysosomal storage disorders and Hepatitis C virus replication.