Lipid-protein interaction in the primary cilium (28*)

初生纤毛是从大多数真核细胞表面长出的膜突起。然而，它们的作用相当神秘。纤毛构成了一个独特的微域，它不仅含有一定的蛋白质谱系和组织，而且还形成了一个独特的膜域，其脂质成分与细胞体不同。然而，脂类如何控制纤毛功能，特别是纤毛中蛋白质的功能，还不是很清楚。我们将使用最先进的成像技术分析神经鞘糖脂和某些磷脂酰肌醇在控制初级纤毛信号(如Hedgehog信号)和纤毛功能中的作用。我们分析脂类如何控制初级纤毛中的蛋白质信号的方法将揭示在生理和病理条件下纤毛功能调节的新见解。

Primary cilia are membrane protrusions, emerging from the surface of most eukaryotic cells. However, their function is rather enigmatic. Cilia constitute a unique microdomain, containing not only a certain protein repertoire and organization, but also forming a distinct membrane domain, whose lipid composition is different from the cell body. However, how lipids control ciliary function, in particular the function of proteins in the cilium, is not well understood. We will analyze the role of the glycosphingolipid glucosylceramide and certain phosphoinositide species in controlling primary ciliary signaling, e.g. Hedgehog signaling, and ciliary function using state-of-the-art imaging techniques. Our approach of analyzing how lipids control protein signaling in the primary cilium will reveal new insights into the regulation of ciliary function under physiological and pathological conditions.