Animal Experimental Model of Human Hepatocellular Carcinoma

解决了土拨鼠的饲养条件，如笼舍、食物等基本问题。我们制作了不锈钢室内笼（O。36平方厘米的空间），这是非常有用的保持动物观察他们或做实验，但在这个笼子里，他们没有交配或繁殖。在较大的室内铁箱（2平方米）中，动物可以繁殖，但新生的幼崽经常被母鼠杀死。因此，最佳的繁殖环境是室外永久性地上笼舍，其产仔数与野生动物相同。我们制作的饲料颗粒与豚鼠饲料颗粒相同，但粗纤维含量较少。我们成功地建立了一种将病毒注射到非病毒携带者幼犬体内获得人工病毒携带者的方法。即使在6周大的小狗，我们也可以得到积极的结果。我们得出结论，2-4周龄的幼犬最好注射病毒，因为它们睁着眼睛非常活跃，死亡率已经很低。新获得的携带者仍然不会发展癌症。我们已经通过RIA检测肝炎病毒表面抗原的阳性，但我们开发了利用聚合酶链反应的新技术。我们用编码表面抗原的DNA片段作为引物，追踪野生型自发携带者检测肝癌，发现当肿瘤体积大于10 cm ^3时，发展非常迅速。病毒DNA的整合模式和n-myc重排表明癌症同时发生多中心和转移。C-myc重排非常罕见。

Basical problems about conditions of care and breeding woodchucks such as cages, housing or food were solved. We made stainless steel indoor cages(O. 36cm^2 floor space) which are very useful for keeping animals to observe them or make experiment but in this cage they did not mate or breed. In larger indoor iron boxes(2 m^2) animals could breed but new born pups were often killed by their dams. In our conclusion the best circumstances for breeding is outdoor permanent above ground cages in which litter size same as those of wild animals were obtained. Food pellets we produced are same as those of guinea pigs containing less crude fibers.We succeeded in establishing a method for obtaining artificial virus carriers by injecting virus into non virus carrier pupps. Even in 6 weeks old pupps we could get positive results. We conclude 2-4 weeks old pupps are best to be injected with virus as they are very active with open eyes and mortality is already low. Newly obtained carriers do not still develop cancers. We have checked positivity of hepatitis virus by RIA for surface antigen but we developed new technique utilizing polymerase chain reaction. As primer we used DNA part coding surface antigen.We followed wild spontaneous carriers for detecting hepatic cnacers and found when volume of cancer become larger than 10cm^3 they develop very rapidly. Integration pattern of virus DNA and n-myc rearrangement suggested that cancer develop both multicentric and metastatic. C-myc rearrangement occur very rare.