Investigating the evolutionary relationships of Arcellinida by single-cell phylogenomics of Phryganella (Arcellinida, Amoebozoa).

单细胞转录组学是建立健全的原生生物系统发育的新的前沿技术。由此产生的丰富数据使其能够直接与微生物的功能多样性联系起来。在变形虫中，形态多样的有壳阿米巴根据形态和单基因系统发育的拼凑分析被统一在Arcellinida目下。甲壳中的特征和信息形态特征在分类学和生态学中具有历史价值，现在它们经常被用作生态和古生态研究的生物指示物。然而，多基因方法揭示了传统的基于形态的分类存在严重的不一致，最近显示出牛目是多系的，这对任何基于传统的基于形态的分类的生态学数据解释提出了挑战。为了进一步研究这一点，我们获得了来自该属三个形态种的5个培养菌株的SSU rDNA序列的初步结果，表明该科的类群是在Arcellinida的基础上进行的。我们得出的结论是，需要用多基因方法来阐明金针藻在变形虫中的系统发育地位。因此，单细胞转录本将导致对这三个培养的形态种进行彻底的分子表征，进一步深入了解ArcellinteState阿米巴的进化，学习这种方法将有助于个人的职业发展，并为我和同事未来的提议和研究奠定基础。

Single cell transcriptomics is the new cutting edge technique to establish sound protist phylogenies. The wealth of data thus generated enables a direct link to the functional diversity of microbes. In Amoebozoa, morphologically diverse shelled amoebae were unified under the order Arcellinida based on morphology and a patchwork of single-gene phylogenetic analyses. The characteristic and informative morphological characters present in the shell of the Arcellinida have been historically useful in taxonomy and ecology, nowadays they are often used as bioindicators in ecological and paleoecological studies. However, multigene approaches reveal severe inconsistencies in the traditional morphology-based classification and have recently shown the Arcellinida to be polyphyletic, challenging any ecological data interpretation based on traditional, morphology-based classification. To further investigate this, we obtained preliminary results from SSU rDNA sequences of five cultured strains from three morphospecies of the genus Phryganella, indicating that the family Phryganellidae groups at the basis of the Arcellinida. We came to the conclusion that multigene approaches are needed to clarify the phylogenetic position of Phryganella within the Amoebozoa. Single cell transcriptomes will thus lead to a thorough molecular characterization of the three cultured morphospecies, give further insight in the evolution of arcellinid testate amoebae and learning this method will facilitate personal career development and build the fundament for future proposals and research of myself and colleagues.