Dorsiventrality in stomatal responses to green light in bifacial leaves: Identification of green light receptor

用显微镜观察了CO_2浓度(100或700ppm)变化对红光或白光下气孔响应的影响，分别放置在含有缓冲液的凝胶上的鸭茅叶片、表皮条和叶肉段上的表皮条上。在红光和白光下，叶片气孔在低/高CO2条件下迅速打开/关闭。在红光下，表皮条状气孔对CO2几乎没有反应。在白光下，它们在低二氧化碳下打开，但在高二氧化碳下几乎不关闭。放置在叶肉上的表皮条上的气孔的反应方式与叶节上的相同。我们还证实了叶肉信号的质外体传递和低CO2条件下气孔的开放依赖于光合作用，而高CO2条件下气孔的关闭不依赖于光合作用。光中叶肉信号的产生很可能依赖于波长。

The effects of changes in CO2 concentration (100 or 700 ppm) on stomatal responses in red or white light were examined microscopically in a leaf segment, an epidermal strip and an epidermal strip placed on a mesophyll segment of Commelina communis, all mounted on abuffer-containing gel. In both red and white light, stomata of the leaf segment opened/closed rapidly at low/high CO2. In red light, epidermal strip stomata barely responded to CO2. In white light, they opened at low CO2, but hardly closed at high CO2. Stomata of the epidermal strip placed on the mesophyll responded in the same manner as those on the leaf segment. We also confirmed that the apoplast transfer of ‘mesophyll signals’ and the stomatal opening at low CO2 are dependent on photosynthesis, whereas the stomatal closure at high CO2 is independent of photosynthesis. It is highly possible that productionof mesophyll signlas in the light depends on the wavelength.