Development of electron microscopy atlas: Fusion with omics and electron microscopic data

透射电子显微镜（TEM）是高分辨率观察细胞器、细胞和组织超微结构的重要手段。然而，它不适合进行综合分析。我构建了TEM图谱，利用高压冷冻（HFP）和TEM技术对模式植物细胞器进行详尽的超微结构分析。拟南芥根和BY2细胞经HFP机冷冻后固定，以获得宽分辨率TEM图像。在树脂中嵌入后，将大型超薄切片放在孔网格上。然后利用瞬变电磁法自动数字记录系统拍摄瞬变电磁法图像。我成功地记录了3万多张照片。我们开发了TEM图像的自动合成程序，实现了数千张图像的自动合成。利用这些高分辨率的图片，我开发了一个基于网络的2D地图，可以放大和搜索。

Transmission electron microscopy (TEM) is important for high-resolution ultrastructure observation for organelles, cell and tissues. However, it is unsuitable for comprehensive analyses. I constructed TEM atlas to achieve an exhaustive ultrastructural analysis of organelles in model plants by using high-pressure freezing (HFP) and TEM techniques. To take wide and high-resolution TEM pictures, Arabidopsis roots and BY2 cells were frozen by HFP machine and then fix. After embedded in resin, large ultrathin sections were put on a hole grid. Then we took TEM pictures by auto-digital TEM recoding system. I succeed in records more than 30 thousand images. We developed auto-photomerge program for TEM pictures and several thousands of pictures were automatically merged. Using these wide-high resolution pictures, I have developed a web-based 2D map that can be zoomed and searched.