Metagenomics using porous arrowhead devices; from environmental assessment to screening

提出了一种多孔材料制成的箭头装置作为环境宏基因组学的工具。利用该装置研究了不同化合物对微生物菌群的影响。此外，通过将筛选对象渗透到该装置中，尝试积累具有特定功能的微生物。结果，我通过逐渐释放目标物质，成功地在这个装置的外围产生了特定的微生物菌群。此外，5厘米左右的箭头距离足以获得良好的效果。因此，这种方法节省空间。这种方法在未来一定是一种廉价、简单的宏基因组技术。

As a tool of environmental metagenomics, an arrowhead device made of porous material was suggested. Impacts against microbial flora of various chemical compounds were investigated by using this device. In addition, accumulation of microorganisms with specific function is tried by infiltrating a screening object into this device. As a result, I succeeded to produce specific microbial flora to the outskirts of this device by gradually releasing a target material from it. Furthermore, around 5cm was enough for the distance of the arrowhead to get good result. Thus this method is space-saving. This method must be a cheap, and simple metagenome technology in future.