Stabilization of the population of sea urchins as the materials for physiological and developmental studies

1.本研究尝试在海洋实验室内，在缺乏养殖设备的条件下，建立受精卵培育幼海胆的方法.结果表明，本实验共获得7种幼海胆数千只，分别为扁棘球海胆（Pseudocentrotus depressus）、厚刺花海胆（Anthocidaris crassispina）、马粪海胆（Hemicentrotus pulcherrimus）、短肋海胆（Temnopleurus toreumaticus）、棘球海胆（Echinostrephus aciculatus）、裸球海胆（Strongylocentrotus nudus）和球海胆（Mespilia globulus）.将Pluteus lavae保持在30升塑料容器中的25升纸过滤海水中（6升/ml）。结果表明，一种硅藻--纤细角毛藻（Chaetoceros gracilis）是该幼虫最适宜的饵料.当幼虫达到8臂阶段并且海胆原基在其中生长足够大时，将它们转移到500升塑料容器中，并给予被硅藻覆盖的塑料板。幼虫立即在平板上开始变态。幼鱼以石莼、马尾藻等海藻为食。当它们变成5-10毫米时，它们被释放到海里。每艘可容纳500只垃圾的船只最多可容纳10，000只幼鱼。

1. We tried to establish the method to rear juvenile sea urchins from fertilized eggs in the marine laboratories where the equipment for aquaculture is not fully available.2. We could obtain several thousands of juvenile sea urchins in 7 species, Pseudocentrotus depressus, Anthocidaris crassispina, Hemicentrotus pulcherrimus, Temnopleurus toreumaticus, Echinostrephus aciculatus, Strongylocentrotus nudus and Mespilia globulus.3. Pluteus lavae were kept in 25 litter paper-filtered sea water in 30 litter plastic vessels (6 plutei/ml). A diatom, Chaetoceros gracilis, was found to be the most suitable food of the larvae.4. When the larvae reached 8-arm stage and the sea urchin primordium grew large enough within them, they were transferred into a 500 litter plastic vessel and given plastic plates which were covered by diatoms. The larvae immediately began metamorphosis on the plates.5. The juveniles were fed by sea weeds such as Ulva and Sargassum. When they became 5-10 mm, they were released into the sea.6. The maximum number of juveniles obtainable from a 500 litter vessel will be 10,000.