Developmental Studies of Eluorescein Video Angiography and New Analysis Software on Retinal Microcirculation

我们开发了一种测定视网膜平均循环时间（MCT）的系统。荧光素视频血管造影术：将超高灵敏度电视摄像机与荧光素照相装置连接。用录像带记录眼底荧光素视频反图。图像分析与计算：将再现的视频血管图像传输到图像分析仪，分析视网膜大血管的密度。测量数据回归到最优对数正态分布函数，显示染料稀释曲线。计算给定视网膜节段的mct。再现性。进行了双重测定。结果表明，该系统具有良好的重复性，可作为临床测量视网膜循环的工具。正常的价值。50只正常眼的MCT为3.00 +/- 1.26（平均值+/标准差）。视网膜循环时间在4个主要视网膜节段间无显著差异。视网膜分支静脉结扎（BRVO）： 10例新鲜BRVO，受累区MCT较未受累区长。22例老年BRVO受累区MCT短于非受累区。糖尿病视网膜病变：测量38只糖尿病眼的MCT。单纯性视网膜病变的MCT较正常人短。增生性视网膜病变患者的MCT时间较正常人延长。此外，增殖性视网膜病变的MCT时间长于增殖性视网膜病变。伯格氏病：一例伯格氏病患者的MCT延长，尽管有侧枝经络存在，提示视网膜循环不足。

We developed a system determining retinal mean circulation tiem(MCT).1. Fulorescein video angiography: A superhigh sensitivity TV camera was connected to a fulorescein photograph unit. The fluorescein video antiogram of the ocular fundus was recorded in a bideo tape.2. Image analysis and calculation: The reproduced bideo angiogram was transferred to an image analyzer and densities of large retinal vessels were analyzed. The measured data were regressed to an optimal lognormal distribution function manidesting a dye dilution curve. MCTs of given retinal segements were calculated.3. Reproducibility. Double determination was performed. It was proved that this system had a good reproducibility as a clinical tool measuring retinal circulation.4. Normal valus. MCT in 50 normal eyes was 3.00 +/- 1.26 (mean +/standard deviation). There were no significant differences in retinal circulation time between four major retinal segments.5. Branch Retinal Vein Cclusion (BRVO): In ten fresh BRVO, MCT in the involved area was longer than in the non-involved area. In 22 old BRVO, MCT in the involved area was shorter than in the non-involved area.6. Diabetic Retinopathy: MCT was measured in 38 diabetic eyes. MCT in the simple retinopathy was shorter than in the noermal. MCT in the proliferative retinopathy was longer than in the normal. Furchermore, MCT in the preproliferative retinopathy was longer than in the proliferative retinopathy.7. Buerger disease: MCT of a patient with Berger disease was prolonged in spite of the presence of collateral channels, indicating insufficiency of the retianla circulation.