Development of a new biotechnological manufacturing process of bifidogenic substances in milk

哈茨木霉在含乳糖培养基中生长时产生半乳糖寡糖，特别是三糖。6′-半乳糖是哈茨木霉产生的主要低聚糖。在活性状态下，从哈茨木霉中分离出的< β - >-半乳糖苷酶具有显著的半乳糖基化活性。本研究中使用的哈茨木霉菌株具有很强的形成< β >(1-6)连锁的能力，这反过来又导致大量生产6'-半乳糖基-乳糖。认为可以对该菌株进行进一步的深入研究，以确定以乳糖为原料大规模生产双歧低聚糖，特别是6′-半乳糖-乳糖的最佳工艺条件。

Trichoderma harzianum produces galacto-oligosaccharides particularly tri- saccharides during growth in lactose containing medium. 6'-Galactosyl-lactgose is the main oligosaccharide formed by Trichoderma harzianum. In its active state, the <beta>-galactosidase isolated from Trichoderma harzianum shows signi- ficant transgalactosylation activity. The Trichoderma harzianum strain used in the presen tinvestigation is ghghly capable of forming <beta>(1-6) linkage which in turn leads to high amount production of 6'-galactlosyl-lactgose. It is thorght that the present strain of Trichoderma harzianum could be further studied critically to formulate optimum process conditions for use in large scale production of bifidogenic oligosaccharides particularly 6'-galactosyl- -lactose from lactose.