Post-transcriptional regulation of phytohormone signal transduction in Arabidopsis immunity

转录后过程最近已被证明在适应蛋白质丰度的植物从生长到免疫的过渡中发挥重要作用。已知植物激素控制这些稳态及其过渡的大多数方面。在这里，我们提出了一个有针对性的多组学方法，主要集中在有针对性的蛋白质组学测量蛋白质周转率。我们将研究SA，JA，ET和生长素相互作用的转录，转录后/翻译和翻译后（蛋白质降解）过程中的时间顺序，以及生长和免疫之间的建立和过渡。我们还将研究丰富的光合和初级代谢酶的降解率的改变是否参与了从生长到免疫的过渡中细胞蛋白质组的整体重塑。这些问题对于理解植物的生长防御权衡是很重要的。

Post-transcriptional processes have recently been shown to play significant roles in adapting protein abundance in transitions from growth to immunity in plants. Phytohormones are known to govern most aspects of these steady states and their transitions. Here we propose a targeted multi-omics approach with a primary focus on targeted proteomics measurements of protein turn-over rates. We will investigate the chronology of transcriptional, post-transcriptional/translational and post-translational (protein degradation) processes of SA, JA, ET and auxin interaction in the establishment and transition of and between growth and immunity. We will also examine if altered degradation rates of abundant photosynthetic and primary metabolic enzymes are involved in remodeling the cellular proteome as a whole in the transition from growth to immunity. These questions are important to an understanding of the growth defense trade-off in plants.