The correlation and contribution of spatiotemporal firing pattern of inferior olive neurons to the cyclical movement

本研究旨在探讨IO神经元的结构和功能集群，并研究IO神经元的时空放电模式与小鼠步行/跑步表现的相关性和贡献。本研究开发的IO记录方法可以应用于其他行为协议，并有利于未来的橄榄-小脑系统相关研究。建立了一个包括跑步机和用于运动研究的摄像机的行为设置。该装置已在小鼠身上进行了测试，以优化速度等参数，以适应小鼠的各种步态。将晶状体植入下橄榄进行钙成像的手术程序已经过测试和优化，以在对动物伤害最小的情况下获得一致的结果。部分初步成果已在神经科学学会年会、日本神经科学学会年会、中国神经科学学会年会等国际会议上分享和讨论，征求同行科学家的意见。

This study aims to explore the proposed structural and functional clusters in IO and investigate the correlation and contribution of spatiotemporal firing patterns of IO neurons to mice walking/running performance. The IO recording method developed in this study can be applied with other behavior protocols and benefit future olivo-cerebellar system-related research.A behavior setup including a treadmill and cameras used for locomotion study has been built. The setup has been tested with mice to optimize the parameters, such as speed, to fit various gaits of mice.The surgical procedure for implantation of the lens into the inferior olive for calcium imaging has been tested and optimized to achieve consistent results with the least damage to the animal.Some preliminary results have been shared and discussed at international conferences, such as the Annual Conference of Society for Neuroscience Annual Conference, The Annual Conference of Japan Neuroscience Society, and The Annual Conference of Chinese Neuroscience Society, for suggestions from peer scientists.