Developmental Study on a Simultaneous Processing System for Microphotometry and Video Image of Dynamic Cell Function.

我们试图建立一个同时进行细胞功能的视频成像和显微测量记录的系统。该系统由一个光学系统和两个检测系统组成，光学部分使用了尼康显微镜(FLUOPHOT MICROLICAL V系列)。在该显微镜中，通过放置在舞台上的制剂的显微镜物镜的光被半镜分成两个方向，并用高S/N比摄像机(Hamamatsu Phodonics，c-1800)和5x5元光电二极管阵列(Centronix，MD-25，Croydon，UK)在每个方向上检测两个分割的光。摄像机的输出被送入(Hamamatsu Photonics，C-1901)的图像处理，并且处理后的图像被传输到计算机系统并显示。计算机程序是用PASCAL语言编写的。另一方面，光电二极管阵列的输出经16路放大器、多路复用器和A/D转换器传输到计算机并显示。我们应用该系统对蛙房标本的自发电兴奋和耦合收缩进行了监测。利用光电二极管阵列和压敏染料检测与电激发相关的光信号，获得了与接触相关的光散射变化的地形图。利用该系统，通过计算机辅助数据处理，成功地实现了激励信号和收缩信号的分离。

We have tried to ccqnststruct a system to simultaneously carry out both the video-imaging and the microphotometric recording of cell functions. The system is composed of an optics and two detection systems.In the part of optics, we used a Nikon microscope (FLUOPHOT microphoto V Series). In this microscope, light via a microscopic objective from preparation placed on the stage was devided in two directions with a half mirror, and the two devided light were detected by a high S/N ratio video camera (Hamamatsu Phodonics,c-1800) and a 5 x 5-element photodiode array (Centronix, MD-25, Croydon,UK) in each direction. An output from the video camera fed into an image processof (Hamamatsu Photonics, C-1901), and the processed image was transfered to a computer system and displayed. The computer programs were written in Pascal language. On the ogher hand, the outputs from the photodiode array were passed to 16- amplifiers, a multiplexer and an A/D converter, and then they were transfered to the computer and displayed.We have applied this system to monitor the spontaneous electrical excitation and coupled contraction of the frog atrium preparations. The electrical excitation-related optical signals were detected using the photodiode array together with voltage-sensitive dyes, and the toporogical images of the contnaction related light scattering changes were obtained. Furthermore, using this system, we succeeded to separate the excitation-signal and contraction-signal by computer-aided data processing.