Coordination Funds

蓝藻中的自养-异养开关：在多个监管层的连贯决策短标题：SCyCode（蓝藻中的开关：连贯决策）蓝藻是地球上原始的氧光合生物。它们卓越的生态影响是基于一种新陈代谢，它将二氧化碳和水转化为有机物质，同时只使用光能释放氧气。最近，蓝藻作为可持续经济的细胞工厂被越来越多地研究。尽管它们在全球环境和经济方面的重要性日益增加，但我们对它们初级代谢调节的了解是碎片化的，这也是由于新陈代谢的不可预见的高度复杂性。蓝藻在昼夜循环或特定环境条件下在光自养和异养代谢模式之间切换，在一个细胞中存在两种生活方式的酶能力。为了破译调节和操作这些过程的分子机制，一个涵盖相关专业知识的跨学科团队通过建立研究单位“蓝藻中的自育异育开关：在多个监管层上的一致决策”（简称“SCyCode”）联合起来。在第一个资助期间取得的重大发现突出了多层调节同工酶的重要性，例如通过氧化还原或能量状态，通过翻译后蛋白质修饰，或通过高阶多酶复合物的动态自组织，其中涉及非酶调节肽，如CP12或新发现的PirC和nld肽。此外，SCyCode已经建立了多亚基蛋白质-代谢物和蛋白质- rna复合物的分析资源，这些资源已经可以通过合适的数据库被社区访问。我们的联盟也开始了对蓝藻磷蛋白质组，代谢组和蛋白质基因组的系统理解的重要步骤。这些资源将在第二个资助期大幅扩展，通过不同的生长条件和生理状态来跟踪主要大分子复合物的组成和修饰的动态变化。根据第一个资助期的结果，我们已经提出了一些假设，我们将在第二个资助期的具体实验中对这些假设进行挑战。一个主要的焦点将是在机制水平上获得的洞察力与途径组织和调节，氧化还原信号和翻译后控制机制的结构功能理解相结合，这将最终形成蓝藻初级代谢的综合动力学模型。这种对负责代谢开关的网络控制的综合观点对于未来的生物技术应用以及对蓝藻代谢及其调节的系统级理解至关重要。

The Autotrophy-Heterotrophy Switch in Cyanobacteria: Coherent Decision-Making at Multiple Regulatory LayersShort title: SCyCode (Switch in Cyanobacteria: Coherent decision-making)Cyanobacteria are the primordial oxygenic photosynthetic organisms on Earth. Their outstanding ecological impact is based on a metabolism, which converts carbon dioxide and water into organic material with the concomitant release of oxygen only using light energy. Recently, cyanobacteria are increasingly investigated as cell factories for a sustainable economy. Despite their global environmental and rising economic importance, our knowledge on the regulation of their primary metabolism is fragmented, which is also due to the unforeseen high complexity of metabolism. Cyanobacteria switch between photoautotrophic and heterotrophic modes of metabolism during day/night cycles or under specific environmental conditions with the enzymatic capacity for both life-styles being present in one cell. To decipher the molecular mechanisms regulating and operating these processes, an interdisciplinary team that covers the relevant expertise joined forces by establishing the research unit “The Autotrophy Heterotrophy Switch in Cyanobacteria: Coherent decision-making on multiple regulatory layers” (abbreviated “SCyCode”). Major discoveries achieved during the first funding period highlight the importance of isoenzymes regulated at multiple layers, for example by the redox or energy state, by post-translational protein modifications, or by the dynamic self-organization of higher order multi-enzyme complexes, which involves non-enzymatic regulatory peptides such as CP12 or the newly discovered PirC and NblD peptides. Furthermore, SCyCode has established resources for the analysis of multi-subunit protein–metabolite and of protein-RNA complexes that can already be accessed by the community through suitable databases. Our consortium has also initiated major steps for the systematic understanding of the cyanobacterial phosphoproteome, metabolome and proteogenome. These resources will be substantially extended during the second funding period by following the dynamic changes in the composition and modification of major macromolecular complexes through different growth conditions and physiological states. Based on the results from the 1st funding period, hypotheses have been developed which we will challenge in specific experiments during the second funding period. A major focus will be combining the obtained insight at mechanistic levels with a structure-function understanding of pathway organization and regulation, redox signalling, and posttranslational control mechanisms, which will culminate in an integrative kinetic model for cyanobacterial primary metabolism. Such an integrated view on the network control responsible for metabolic switches is essential for future biotechnological applications as well as a system level understanding of cyanobacterial metabolism and its regulation.