注射可能な吸収性ゲルのドラッグデリバリーシステムの開発－低侵襲の新規骨造成法

注意事项：为了达到理想的功能和美学要求，在种植牙之前通常需要进行牙槽嵴增高。通常由复杂和侵入性手术组成的骨增量（特别是垂直骨增量）仍然是一个挑战。本研究旨在探讨含锶羟基磷灰石（SrHA）和藻酸盐（Alg）的可注射原位凝胶形成系统的可行性，并评估在SrHA-Alg系统中添加辛伐他汀（SIM）作为刺激剂以进一步增强微创方式的骨形成。材料和方法：根据先前的方案用低、中、高剂量的SIM制备SrHA-Alg-SIM溶液，每只动物接受200 μL单一骨膜下注射的SrHA-Alg-SIM溶液或单独的SrHA-Alg溶液作为对照。结果：锶羟基磷灰石-藻酸盐溶液材料注射后可形成固体凝胶。因此，无需缝合来闭合注射部位。显着更大量的新骨形成中观察到的SrHA-Alg-SIM（低剂量）组与其他组相比，无论是通过micro-CT和组织学section.CONCLUSIONS：大鼠颅骨骨增量实现了由一个单一的骨膜下注射的SrHA-Alg-SIM解决方案，而不需要任何管理的干细胞或生长因子。低剂量辛伐他汀对骨形成的促进作用最好。原位凝胶形成材料可以以微创方式保持局部骨增量的潜在治疗益处。

OBJECTIVES : To achieve ideal functional and aesthetic requirements, ridge augmentation is often required before dental implant placement. Bone augmentation (especially vertical), which normally consists of complex and invasive surgeries, still remains challenge. This study seeks to investigate the feasibility of an injectable in situ gel-forming system containing strontium hydroxyapatite (SrHA) and alginate (Alg) and also evaluate the addition of simvastatin (SIM) in the SrHA-Alg system as a stimulant to further enhance bone formation in the minimally invasive manner.MATERIAL AND METHODS : SrHA-Alg-SIM solution was prepared with low, medium and high doses of SIM according to the previous protocol, and each animal received a 200-μL single subperiosteal injection of either SrHA-Alg-SIM solution or SrHA-Alg solution alone as control. The new bone formation was assessed at 0, 4, and 8 weeks histologically and radiologically.RESULTS : The SrHA-alginate solution materials could form solid gel once injected. As such, no sutures were required to close the injection site. Significantly greater amount of new bone formation was observed in the SrHA-Alg-SIM (Low dose) group compared with the other groups both by micro-CT and by histological section.CONCLUSIONS : Rat calvarial bone augmentation was achieved by a single subperiosteal injection of SrHA-Alg-SIM solution without any administration of stem cells or growth factors. The low dose of simvastatin incorporated seemed to have the best stimulatory effect in bone formation. The in situ gel-forming material may hold potential therapeutic benefits for local bone augmentation in a minimally invasive manner.