Regulation of plant chromatin organization by lamin proteins

核膜不仅是将核内容物与细胞质隔开的物理屏障，而且在调节基因组DNA的三维结构中起着至关重要的作用。对于动植物来说，核周是一个富含异染色质的功能区。到目前为止，植物NP如何与染色质相互作用尚不清楚。在我们最近的报告显示了拟南芥染色质与NP之间的全基因组相互作用之后，我们发现需要一种植物层蛋白(CRWN1)来将染色质与NP捆绑在一起。我们将重点放在这个蛋白质上，在这里我们建议努力了解它是如何调节染色质活性的。我们在这个项目中提出了三个部分，在第一部分，我们将使用遗传学方法来鉴定与CRWN1相互作用的染色质沉默途径(S)。在第二部分中，我们计划使用各种分子方法来研究这种Lamin蛋白如何与染色质和其他染色质调节因子相互作用。在第三部分中，我们将使用生物信息学的方法来描述突变植物染色质结构和表观遗传景观的变化，并分析这些变化是如何与基因转录联系在一起的。综上所述，本项目旨在首次对植物NP-染色质相互作用进行功能研究，这将极大地促进我们对植物Lamins在调节染色质活性中的作用的理解。

The nuclear envelope not only serves as a physical barrier separating nuclear content from the cytoplasm but also plays critical roles in modulating the three-dimensional organization of genomic DNA. For both plants and animals, the nuclear periphery (NP) is a functional compartment enriched for heterochromatin. To date, how plant NP interacts with chromatin is unclear. Following our recent report showing genome-wide interactions between chromatin and the NP in Arabidopsis, we found that a plant lamin protein (CRWN1) is required to tether chromatin to the NP. With a focus on this protein, here we propose work towards understanding how it regulates chromatin activity. We propose three parts in this project, in the first part, we will employ genetic approaches to identify chromatin silencing pathway(s) interacting with CRWN1. In the second part, we plan to use various molecular methods to study how this lamin protein interacts with chromatin and other chromatin regulators. In the third part, we will use bioinformatic approaches to profile changes in chromatin structure and epigenetic landscape in mutant plants and to analyze how these changes are linked to gene transcription. Altogether, this project aims to present the first functional study of plant NP-chromatin interactions, which will substantially advance our understanding of the role of plant lamins in regulating chromatin activity.