Enzyme cooperativity in the biosynthesis of antibiotics and their cellular target structures (B06)

酶的协同性对许多生物合成过程都很重要。在本项目中，酶的协同性将研究抗生素的各种生物合成机制或其细胞靶结构。我们将研究协同性是如何在细胞中组织的，重点关注三个主要系统中酶之间的相互作用：1)PKS/NRPS生物合成中的磷酸antetheinyl转移酶和载体蛋白，2)萜烯生物合成中的细胞色素P450和氧化还原伙伴蛋白，以及3)细胞壁生物合成中的丙氨酸消旋酶和D-Ala-D-Ala连接酶。

The cooperativity of enzymes is important for many biosynthetic processes. In this project, enzyme cooperativity will be studied for various biosynthetic machineries of antibiotics or their cellular target structures. We will investigate how cooperativity is organized in the cell focusing on the interaction between enzymes in three main systems: 1) phosphopantetheinyl transferases and carrier proteins in PKS/NRPS biosynthesis, 2) cytochromes P450 and redox partner proteins in terpene biosynthesis, and 3) alanine racemase and D-Ala-D-Ala ligase in cell wall biosynthesis.