Effect of Campylobacter Pylori upon Immunological Conditions of Chronic Gastritis.

幽门螺杆菌或幽门螺杆菌最近被认为是胃十二指肠疾病的病因。Western blot法将患者血清中幽门螺杆菌抗体与正常人进行比较，在患者血清中检测到一种独特的抗体，该抗体能与25千分子质量的幽门螺杆菌抗原反应。另一方面，本实验室制备的单临床抗体CP3也能识别分子量为25 Kilo的幽门螺杆菌抗原。然后检测患者血清抗体是否识别CP3单临床抗体纯化的CP3抗原。Western blot分析表明，患者血清与亲和纯化的CP3抗原有强烈反应。采用单临床抗体CP3，建立了酶联免疫吸附法检测血清中CP3抗体。慢性胃炎和胃溃疡患者CP3抗体滴度明显高于正常对照组，且与胃窦性胃炎的组织学分级相关。血清CP3抗体的检测对诊断幽门螺杆菌感染相关的慢性胃炎和胃溃疡及评价胃炎的分级具有重要意义。

Campylobacter pylori or Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to Helicobacter pylori in the sera of patients to that of normal controls by Western blot, a unique antibody was detected in the sera of patients, which reacted with the 25 Kilo molecular weight antigen of Helicobacter pylori. On the other hand, monoclinal antibody CP3 which was prepared in our laboratory also recognized the 25 Kilo molecular weight antigen of Helicobacter pylori. We then examined whether the serum antibody of the patient recognized the CP3 antigen purified by monoclinal antibody CP3. Western blot analysis demonstrated that the patient's serum strongly reacted with the affinity-purified CP3 antigen. By using monoclinal antibody CP3, We have established an enzyme-linked immunosorbent assay to detect CP3 antibody in sera. In chronic gastritis and gastric ulcer patients, the titer of CP3 antibody was significantly higher than that of normal controls and correlated with the histological grade of antral gastritis. The detection of CP3 antibody in sera is useful to diagnose chronic gastritis and gastric ulcer associated with Helicobacter pylori infection and also to evaluate the grade of gastritis.