Study of regulation of follicle development and oocyte maturation

1. 随着卵泡的成熟，从睾酮到雌激素的芳香酶活性增加。在卵泡液中，存在一种促性腺激素抑制剂，其活性随着卵母细胞的成熟而增加。芳香酶抑制剂显著抑制颗粒细胞DNA合成。卵泡中卵母细胞的葡萄糖含量随着卵母细胞和谷粒细胞之间的间隙连接的减少而减少。5 .受精后，葡萄糖含量逐渐增加至4细胞期，8细胞期后急剧增加。蔗糖处理后36 h，蔗糖处理胚的葡萄糖含量低于对照。7 .透明果破裂对处理后20小时胚胎己糖激酶和G6PDH活性无影响。在大鼠卵巢中，卵泡数量迅速增加，直到21天大。21天后，卵泡直径是卵巢增大的主要原因。

1. As follicles become maturer, aromatase activity from testosterone to estrogen increased.2. In follicular fluid, there exists a gonadotropin inhibitor, whose activity increases as oocytes become maturer.3. Aromatase inhibitor markedly suppressed DNA syntheses in granulosa cells.4. The amount of glucose of an oocyte in a follicle decreases as the gap junctions between an oocyte and glanulosa cells decreases.5. After fertilization, the glucose content increase gradually up to 4-cell-stage and increases vigorously after 8-cell-stage.6. The glucose content in the embryo exposed to sucrose was lower than that in the control 36 hours after sucrose exposure.7. The crack in gona pellucida exercises no influence on hexokinase and G6PDH activities of embryos 20 hours after the treatment.8. In the rat ovary, the number of follicles rapidly increase up to 21 days old. After 21 days, follicle diameter is mainly responsible for the ovarian enlargement.