Effects of the Periodontal Implant Materials on the Early Stage of Mineralization of Osteoblast-LIKE CELLS

我们估计了体外和体内实验系统，以了解人工骨植入材料的确切效果。本研究采用集落分离的方法从小鼠颅骨中分离成骨细胞。这些细胞具有成骨细胞的功能。为了评估植入材料在成骨细胞中的生物活性和钙化程度，我们将细胞和植入材料分别培养21天。培养21天后，羟基磷灰石（HAP）和真骨陶瓷（TBC：牛烧结骨）对这些细胞表现出生物亲和性。但TBC周围较其他材料多出现新钙化迹象。在此基础上，我们考察了胶原蛋白涂层对促进修复和早期钙化的附加作用。培养TBC和胶原包被TBC (C-TBC) 21天。结果表明，用胶原蛋白包覆材料后，成骨细胞有较好的修复和早期钙化。在人工种植材料植入后的实验性骨缺损中，组织学上观察到新生骨的形成，但新生骨的成熟程度并不能由此判断。因此，测量钙和磷的密度可以用来检查骨形成和周围的种植材料。在三个实验中，我们校准了计算机辅助微量分析仪（CMA）来测量Ca和p。该仪器由电子探针微量分析仪（EPMA）和计算机图像分析仪组成。手术后用HAP、TBC或C-TBC填充缺陷。对照组未行种植。分别在1个月和3个月后处死两只狗，这些结果表明，可以确定其他移植体与植入材料的距离。在三个月的标本中，CMA测量显示TBC和C-TBC种植体周围区域具有高密度的Ca和P。

We have estimated in vitro and in vivo assay system to know the precise effects of artificial bone implant materials. In this study, we isolated osteoblastic cell from mouse calvaria using colony separation. These cells ratain osteoblastic functions. To estimate the biological activity and calcification of implant materials in osteoblastic cells, cells and implant materials were cultured for 21days. After 21days cultivation, hydroxyapatite (HAP) and True Bone Ceramic (TBC : bovine sintered bone) showed the biological affinity on these cells. However the around of TBC showed newly calcified evidence more than other materials. Addition of this study, we examined about the additional effect of promoting the mending and early calcification by coating the materials with collagen. We cultivatied TBC and collagen-coated TBC (C-TBC) for 21 days. From these results, coating the materials with collagen, the osteoblastic cells show better mending and early calcification. New bone formation was observed histologically in experimental osseous defects after implantation of artificial implant material, but the degree of maturation of this newly formed bone could not be determined from such observation. Therefore measurement of the density of Ca and P can be used to examine bone formation and around implant materials. In three experiments, we calibrated a computer-aided micro analyzer (CMA) to measure Ca and P. This devise is composed of an electron probe microanalyzer (EPMA) and a computed image analyzer. After the operation the defects were filled with HAP, TBC or C-TBC. No implantation was performed the control side. After one month and three month, the two dogs were sacrificed and these results show that, it is possible to determine the distance from implant materials of other tis sue. In the three months specimens, CMA measurement showed the area surrounding the TBC and C-TBC implants to have a high density of Ca and P.