On the Multiplication and Antigen in Variants of the NPV OF Silkworm, Bombyx mori.,

家蚕核型多角体病毒（BmNPV）存在多种变异。因此，对变异体之间的颗粒形状和大小、多角体的形状和蛋白质以及病毒体的结构蛋白和DNA进行了比较研究。1.与实验室中传代的BmNPV的病毒体一样，颗粒是杆状的，当被包裹时测量约98- 140 nm宽和350- 433 nm长。2. BmNPV的病毒粒子为单衣壳型，通常一个核衣壳被单个囊膜包裹，偶尔也有两个核衣壳被包裹在一个囊膜内，而La株为多衣壳型，一个囊膜内包裹2 ~ 5个粒子。3.从感染NPV的中肠细胞或培养细胞中出芽的病毒粒子，其核衣壳呈弯曲的U形，被宿主细胞质膜包裹。4.用骨髓瘤细胞融合法制备了抗BmNPV Ⅰ株病毒粒子的单克隆抗体。用直接抗原阴性染色法检测I株包膜特异性抗原的存在。5. C-菌株可以在BmN-培养细胞中进行最多的复制。6.用Hind Ⅲ、EcoRI、BanHI、Kpn Ⅰ和Sma Ⅰ酶切λ噬菌体DNA，测定了T、Ta、C和54 N 4株病毒DNA的限制性片段大小。C株与54 N株、T株与Ta株的碱基序列相似。7.采用SDS-PAGE电泳法对T、Ta、C和54 N菌株的多角体蛋白进行电泳分析。T株可能是由于多角体蛋白的57.6KDa多肽缺失所致。

It was found that the nuclear polyhedrosis virus of silkworm(BmNPV) exists many variants. Therefore, shape and size of particles, shape and proteins of polyhedra and structural proteins and DNA of virions were investigated comparatively between variants. 1. As with virions of passaged BmNPVs at the laboratory, the particles are rod shaped, measuring about 98-140nm wide and 350-433nm long when enveloped. 2. The virion of BmNPV is the single capsid type that a nucleocapsid is usually enveloped singly though occasionally two nucleocapsids occurred in one envelope, but La-strain is the multi-capsid type to enveloped 2 to 5 particles in enveloped. 3. Virions budding fropm midgut cells or culture cells infected with NPV showed the structural feature that nucleocapsid is bending U-shape and enveloping with plasma membrane of host cell. 4. A monoclonal antibody has been produced against virion of BmNPV I-strain by the fusion of myeloma cells. The presence of I-strain's envelope specific antigens was detected by the direct antigen negative staining method. 5. C-strain may be carried out most replicate in BmN-culture cells. 6. The size of restriction fragements of viral DNA of 4 strains (T, Ta, C and 54N) were determined with reference to phage lamda DNA digested with HindIII, EcoRI, BanHI, KpnI and SmaI. Similarities of base sequences were found between C and 54N, and between T and Ta strains, respectively. 7. Polyhedral proteins of T, Ta, C and 54N strains were electerphoresed on SDS- PAGE method. The T-strain may be attributed to the deletion of 57.6KDa polypeptied of the polyhedral protein.