PlasNOW – Transport of biologically relevant molecules from the plasma discharge to the biological target in controlled (humid) environments

该项目旨在进一步了解两种不同的非热大气压等离子体对生物组织（如皮肤）的化学和物理影响，以及其功效和促进健康的效果。一氧化氮被认为是人体伤口愈合最重要的触发物质，尤其是皮肤组织。除了一氧化氮本身，一氧化氮衍生物（NODs）如硝酸盐、亚硝酸盐和s-亚硝基蛋白也起着关键作用，特别是对代谢的长期影响。因此，我们一方面希望通过量化NO和NODs从气相到液体的路径来关注NO，另一方面关注受控大气中湿度的影响，因为湿度在大气和人体中都是一次性的。从而影响O、OH和其他物质的浓度。由于等离子体/大气/流体/生物分子系统的复杂性，必须对参数进行控制和调整。因此，我们将采用DBD（介质阻挡放电）和μAPPJ（微大气压等离子体射流），因为它们几乎涵盖了等离子体应用的全部范围：直接处理与间接处理，区域处理与局部处理以及在空气中与在受控（惰性）气体混合物中操作。为了回答以下研究问题，我们限制了参数范围：•NO和NODs的浓度、分布和通量是如何通过液体从源输送/发展到最终目标-生物分子的？什么是流程链？•血浆中NO的生成如何影响到皮肤组织的通量？湿度，特别是OH对NO及其衍生物的产生或损失有什么影响？•产生O或OH等物质的环境参数（如湿度或流体成分）如何影响这些特性？•通过调整气体混合物和外部电气参数，可以在多大程度上优化两种等离子体设备的NO(D)和OH的生产？为此，我们将与合作伙伴合作，在一个共享的定制容器内定制和研究设备的输出，该容器允许两个设备在可变大气中运行。为了克服在液气界面和液体内部测量的众所周知的困难，我们将采用联合努力。我们将在专门的探针中使用生物传感器，该探针也可以引入我们的项目合作伙伴（Metzler-Nolte）的分析系统。此外，我们希望开发一种基于光纤的光谱诊断技术，可以在高空间分辨率的液体中进行检测。我们的第二个合作伙伴（Christoph Suschek）正在研究这些量化分子对活组织的影响。

The project is aimed at gaining further insight into the chemical and physical impact of two different non-thermal atmospheric pressure plasmas on biological tissue, such as skin with respect to the efficacy and health-promoting effect.NO is known to be the most important trigger species for wound healing in the human body and in particular the skin tissue. Despite NO itself, NO derivates (NODs) such as nitrate, nitrite, and s-nitroso proteins play a key role, especially for long-term effects on the metabolism. Therefore, we want to focus on the one hand on the NO path by quantifying NO and NODs from the gas phase to the liquid and on the other hand on the influence of humidity in controlled atmosphere, since humidity is ever disposable in the atmosphere and the human body. Thereby it is influencing the concentration of O, OH and other species. For the complexity of the system of plasma/atmosphere/fluid/biomolecules parameters have to be controlled and adjusted. Hence, we will apply the DBD (dielectric barrier discharge) and the μAPPJ (micro atmospheric pressure plasma jet) since they cover nearly the full range of plasma application: direct vs. indirect treatment, areal vs. localized treatment and operation in air vs. in controlled (noble)gas mixture. We limit the parameter range to answer the following research questions:• How are the NO and NODs concentrations, distributions and fluxes transported/developed from the source over the liquid to the final target – to the bio-molecule? What is the process chain?• How does the NO generation in the plasma influence the flux to the skin tissue? What is the impact of humidity and especially OH on the production or loss of NO and its derivatives?• How do environmental parameters, such as humidity or fluid composition, yielding to species as O or OH influence these properties?• By tuning gas mixture and external electric parameters, to which extend can the production of NO(D) and OH be optimized for both plasma devices?For this, we will tailor and investigate the output of the devices in cooperation with our partners within a shared customized vessel that allows the operation of both devices in variable atmospheres. To overcome the well-known difficulties of measuring at the liquid gas interface and within the liquid, we will use a combined effort. We will use bio-sensors in a specialized probe, that can also be introduced into the analytic systems of our project partner (Metzler-Nolte). Additionally, we want to develop a fiber based spectroscopic diagnostics that allows detection in liquid with high spatial resolution. Our second cooperation partner (Christoph Suschek) is investigation the effects of these quantified molecules on living tissue.