Characterization of novel dwarfing substances A-2alpha and beta, and their application to horticulture

为了鉴定新的生长抑制物质A-2，α和β对矮生豌豆的光抑制作用。为了找到在园艺上的应用，尝试从田间生长的进步豌豆嫩枝中分离它们。虽然A-2‘S的纯分离因其极不稳定而不成功，但获得了许多对大规模提取纯化以及培养和贮藏豌豆芽有用的信息如下：无论培养季节、培养时期，因此不同形状的豌豆芽，主芽和侧芽衍生的芽作为提取材料；采收后立即冷冻的芽是必要的，在-20ﾟC下贮藏多年保持抑制物含量不变。此外，我们还成功地在反相高效液相色谱图上定位了A-2α、β和α以及A-3‘S和A-1-3’S，为分离过程中抑制剂的检测和定量提供了重要依据。我们发现了新的抑制剂，命名为A-1-3α和β，并成功地鉴定了它们的高效液相色谱峰。A-1-3α和β在溶液中是互不相同的异构体。

In order to characterize novel growth inhibitors A-2 alpha and beta responsible for light growth inhibition in the dwarf pea cv. Progress No. 9 and to find an application to horticulture, their isolation was attempted from Progress pea shoots grown in the field. Although an pure isolation of A-2's was not successful because of their extreme instability, many pieces of information useful for a large-scale extraction and purification as well as for culture and storage of pea shoots were obtained as follows: irrespective of the culture seasons, culture periods, and hence of the different shapes of shoots, the main shoot as well as the shoots derived from lateral buds serve as extraction materials; freezing shoots immediately after harvesting is necessary, and storage at -20ﾟ C keeps the inhibitor content unchanged for years. Also, we succeeded in locating A-2 alpha,beta and alpha and A-3's as well as A-1-3's on reverse phase HPLC chromatograms, which serve greatly for detection and quantification of the inhibitors in isolation processes. We found novel inhibitors named A-1-3 alpha and beta, and succeeded in identifying their HPLC peaks. A-1-3 alpha and beta were found to be isomers which change from one another in solution.