Study of Facial Palsy due to Herpes Virus Infection

1. 单纯疱疹病毒致面瘫实验动物模型本研究通过在小鼠耳廓或舌部接种单纯疱疹病毒，成功地制造了模拟贝尔麻痹的急性和短暂性面瘫动物模型。病毒接种后6 ~ 9 d出现面瘫，持续3 ~ 7 d后自行恢复。病理组织学表现为严重的神经肿胀、炎性细胞浸润及空泡变性。这些发现提示病毒性神经炎，与贝尔氏麻痹早期的组织病理学表现相似。面神经和细胞核中也检出HSV抗原。我们还通过共培养的方法证实了HSV在膝状神经节中存在潜伏感染。下一步，我们正试图重新激活这种病毒，并制造一种面部瘫痪的动物模型。采用补体固定试验（CF）和酶联免疫吸附试验（ELIZA）对30例贝尔麻痹患者和11例拉姆齐·亨特综合征患者进行血清学研究。11例患者中有8例显示急性期和恢复期标本间VZV抗体滴度显著升高。然而，没有一例贝尔氏麻痹患者在急性期和恢复期之间表现出HSV抗体滴度的显著上升，尽管贝尔氏麻痹患者的HSV抗体滴度阳性百分比高于匹配的对照组，尽管很少有急性期和恢复期标本的滴度显著上升。

1. Experimental animal model of facial palsy induced byherpes simplex virusIn the present study we succeeded in producing an acute and transient facial paralysis simulating Bell's palsy by inoculating HSV to the auricle or tongue of mice. The facial paralysis developed between 6 to 9 days after virus inoculation and continued 3 to 7 days, and then recovered spontaneously. Histopathologically, severe nerve swelling, inflammatory cell infiltration and vacuolar degeneration were manifested in the affected facial nerve. These findings indicated viral neuritis and were similar to the histopathology demonstrated in early stage of Bell's palsy. HSV antigens were also detected in the facial nerve and nucleus. We also confirmed the presence of latent infection of HSV in the geniculate ganglion by cocultivation method. As a next step, we are now attempting to reactivate this viruses and to make an animal model of facial paralysis.2. Clinical study for Bell's palsy and Ramsey Hunt syndromeSerologic studies have conducted in 30 patients with Bell's palsy and 11 patients with Ramsey Hunt syndrome by complement fixation test(CF) and enzyme-linked immunosorbent assay (ELIZA). Eight of 11 patients demonstrated significant rise in VZV antibody titer between acute and convalescent phase specimens. However, none of patient with Bell's palsy showed a significant rise in HSV antibody titer between acute and convalescent phase, even though higher percentage of positive HSV antibody titer were demonstrated in patients with Bell's palsy than matched controls, even though few showed significant rise in titer between acute and convalescent phase specimens.