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Precise cliagnosis and treatment of facial nerve palsy(Bell's palsy and Ramsay hunt syndrome)

面神经麻痹（贝尔麻痹、拉姆齐亨特综合征）的精准诊断和治疗

基本信息

批准号：
16390491
负责人：
MURAKAMI Shingo
金额：
$ 7.04万
依托单位：
NAGOYA CITY UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390491/
关键词：
facial nerve palsy herpes simplex virus type 1 steroid edaravone hepato growth factor LacZ Bell麻痺 Hunt症候群早期診断病因・病態治療 PCR

项目摘要

There is little study concerning optimum dosage of steroid for Bell's palsy and Ramsay Hunt syndrome. In the present study, we performed aninal study of dose dependent effect of steroid for facial nerve paralysis induced by herpes simplex type 1 (HSV-1). Moreover, we studied the effect of new radical scavenger of edaravone for facial nerve paralysis induced by HSV-1. Steroid was effective for facial nerve paralysis in both clinical, electrophysiological and histopathologically. On the other hand, radical scavenger of edaravone did not show any effect for the recovery of facial nerve paralysis but increased incidence of facial nerve paralysis.Using HSV-1 vector, effects of nerve growth factor for facial nerve paralysis was examined. At first, LacZ was introduced into HSV-1 genomes and the virus was inoculated onto auricle of mice. Expression of LacZ could not find in the intratemporal facial nerve. However, when HSV-1 introduced LacZ was inoculated directory onto the intratemporal facial nerve, LacZ was identified in the intratemporal facial nerve. Then, HGF was introduced into HSV-1 and inoculated it directory onto the intratemporal facial nerve. After 3 weeks, effect of HGF was confirmed in both electrophysiologically and histopathologically. These results suggest that HSV-1 can be useful as a vector for nerve growth factors.

关于Bell麻痹和Ramsay Hunt综合征的最佳类固醇剂量的研究很少。本研究对激素治疗单纯疱疹病毒1型（HSV-1）所致面神经麻痹的剂量依赖性作用进行了动物实验研究。此外，我们还研究了新型自由基清除剂依达拉奉对HSV-1所致面神经麻痹的治疗作用。激素治疗面神经麻痹在临床、电生理和组织病理学上均有效。另一方面，自由基清除剂依达拉奉对面神经麻痹的恢复没有任何作用，但增加了面神经麻痹的发生率。首先，将LacZ引入单纯疱疹病毒1型基因组中，并将病毒接种到小鼠耳廓上。LacZ在颞内面神经中未见表达。而将HSV-1导入LacZ直接接种于颞内面神经后，可在颞内面神经中检测到LacZ。然后将HGF导入HSV-1中，直接接种于颞内面神经。3周后，在电生理学和组织病理学上证实了HGF的作用。这些结果表明，HSV-1可用作神经生长因子的载体。