Molecular Spectroscopic Study on Adhesion Mechanism between Tooth Substrate and Adhesive Resins by Laser Raman Technique.

采用先进的拉曼光谱和红外光谱对牙釉质、牙本质或金属粘接树脂在牙科临床应用中的分子水平进行了研究。研究结果如下：牙科粘接树脂(4-MET/MMA-TBB)与人牙本质界面的拉曼光谱及显微分析；在牙本质侧，直到表面下6微米才能检测到树脂单体。这是首次通过扫描电子显微镜观察到树脂渗入牙本质层的光谱证据。还发现功能单体4-MET在牙体中高度浓缩，详细研究了4-MET与羟基磷灰石混合物的拉曼光谱，并检测到了4-MET的钙盐。然而，4-MET分子很难通过在羧基上形成钙盐连接到HAP上，同时通过在甲基丙烯酸基上与MMA(碱单体)形成共聚物而连接到树脂上。在树脂与牙釉质或牙本质的粘接界面上还检测到了钙盐的存在。用自组装法或旋涂法从MMA溶液中将金属粘结剂底漆吸附在贵金属&GT、M10P和M10PS上，并测定了它们的红外反射吸收光谱。结果表明，这两种单体均以-O-PO_3和lt；2-&gt；等电离形式吸附在贵金属表面，在金衬底上的吸附似乎不同。

Advanced Raman and IR spectroscopic study were undertaken to obtain the molecular level informations about adhesive resins to enamel, dentin or metal in dental clinical use. The results are as follows :<Micro-analysis on the interface between dental adhesive resin (4-MET/MMA-TBB) and human dentin by Raman spectroscopy>Resin monomer was detected until 6mum below the surface in dentin side. This is the first spectroscopic evidence of resin-infiltrated dentin layr which was reported by SEM observation. It was also found that the functional monomer 4-MET was highly condensed in the layr.<The contribution of chemical interaction between dental adhesive resin and tooth substrate>Raman spectra of mixture of 4-MET and hydroxyapatite was investigated in detail and calcium salt of 4-MET was detected. However it was shown to be difficult that a 4-MET molecule is attached to HAP by forming Ca salt at the carboxyl group and is simultaneously linked to resin by forming a copolymer with MMA (base monomer) at the methacryl group. Calcium salt was also detected on the adhesive interface between resin and enamel or dentin.<Adsorption structure of metal adhesive primer to precious metal>M10P and M10PS were adorbed on silver or gold substrate by self-assembly method or spin-coated method from MMA solution and the infrared reflection absorption spectra were mea-sured. It was found that both monomers are adsorbed on the precious metals at their phos-phate groups, which are in the ionized form, -O-PO_3^<2->.On gold substrate, the monomers seem to be adsorbed differently.