Chemical studies of floral bud forming substances

叶片分泌物来自短日照(花诱导)和长日照处理的烟草植株。前者短日照处理的渗出物表现出促进花芽形成的活性，而后者则没有。通过连续柱层析对短日照处理烟叶渗出物中的活性物质进行了纯化。最后，得到了在0.0002 ppm具有刺激活性的少量但很有活性的组份。纯化的活性物质为水溶性物质，具有基本性质，根据透析实验技术，其相对分子质量必须在1000以下。切叶后立即用EDTA-2Na处理叶柄的切面，收集的分泌物中含有另一种相对分子质量更高的活性组分。

The leaf exudate was obtained from short day-treated (flower-induced) and long day-treated tobacco plants. The former short day-treated exudate exhibited flower bud formation-stimulating activity but the latter did not. The active substance contained in the exudate from short day-treated tobacco leaves was purified through successive column chromatographies. Finally, the minor amout but very active fraction exhibiting stimulating activity at 0.0002 ppm has been obtained. The active substance thus purified is water-soluble, showing a basic property, and the molecular weight must be under 1000 from dialysis experiment technique. The cut surface of the petiolets was treated with EDTA 2Na immediately after cutting, and, the exudates then collected contained another active fraction with much higher molecular weight.