Analysis of transformation mechanism from differentiated to undifferentiated thyroid cancer

1. 基因水平分析研究了未分化（间变性）和分化（乳头状）甲状腺癌中p53基因的突变。异常碱基序列仅在间变性癌标本中发现。提示p53基因突变可能引发甲状腺乳头状癌的间变性。间变性甲状腺癌伴分化甲状腺癌患者颈部淋巴结制备人单克隆抗体蛋白水平分析。该单克隆抗体识别的抗原在分化型甲状腺癌中也有表达。该单克隆抗体可能是研究甲状腺癌间变性转化机制的有用工具。细胞动力学分析建立了4株间变性甲状腺癌细胞和3株低分化甲状腺癌细胞。K-199是一种间变性癌细胞系，其分泌的粒细胞集落刺激因子（G-CSF）可能以自分泌或旁分泌机制在甲状腺间变性癌的生长过程中发挥一定作用。通过添加同事提供的分化甲状腺癌细胞系，我们可以制备一个覆盖高分化、低分化和未分化甲状腺癌的细胞面板，包括其他研究者提供的分化甲状腺癌细胞系，我们可以制备从分化到低分化和进一步未分化的甲状腺癌细胞面板。遗传学研究显示，在该细胞组中也发现p53相关间变性的异常表达。

1. Analysis of genetic levelMutation of p53 gene was studied for undifferentiated (anaplastic) and differentiated (papillary) thyroid carcinoma. An abnormal basic sequence was found only in anaplastic cancer specimens. This result suggests that mutation of p53 may trigger the anaplastic transformation from the papillary thyroid cancer.2. Analysis of protein levelHuman monoclonal antibody prepared from cervical lymph nodes taken from patients with anaplastic thyroid cancer with differentiated thyroid cancer specimens. Antigen recognized by this monoclonal antibody was even expressed in differentiated thyroid cancer. This monoclonal antibody may be useful tool for studying the mechanism of anaplastic transformation of thyroid cancer.3. Analysis of cell kineticsFour anaplastic thyroid cancer cell lines and 3 poorly differentiated cancer cell lines were established. K-199, an anaplastic cancer cell line, secretes granulocyte-colony stimulating factor (G-CSF), which may play some role in the growth of anaplastic thyroid cancer with an autocrine or paracrine mechanism. By adding a differentiated thyroid cancer cell line provided by fellow investigators, we were able to prepare a cell panel, which covered both highly and poorly differentiated and also undifferentiated thyroid cancer, ncluding differentiated thyroid cancer cell line, presented by other investigator, we can prepare the cell panel of thyroid cancer covering from differentiated to poorly and further undifferentiated thyroid cancer. As shown in genetic study, abnormal expression of p53 relationg anaplastic transformation was also found in this cell panel.