Immunohistchemical and molecular biological studies on severe mucosal pathology of the upper airway.

1.建立了人呼吸道上皮细胞生长分化为纤毛细胞和分泌细胞的体外培养体系。利用这些漂浮培养的细胞，研究了某些细胞因子对细胞分化的调控以及外源性ATP、内毒素和大环内酯类抗生素引起的纤毛异时性。2.免疫组织化学和生化结果显示，IL-1β是慢性鼻窦炎鼻窦液中的主要细胞因子，由中性粒细胞产生，诱导内皮细胞表达ICAM-1和ELAM-1。3.凝集素组织化学显示，鼻窦炎黏膜中岩藻糖、唾液酸等糖蛋白的染色增强，这些末端碳水化合物的组成不同，甘露糖糖链强度明显降低。高分化型喉鳞癌PNA阳性率高，间变型喉鳞癌SNA阳性率高。4.复发性呼吸道乳头状瘤病(RAP)以HPV-11为主。血清学检测表明，RAP患者存在针对HPV-11亚克隆的重组E6和L2蛋白的抗体。HPV-16的E6和E7基因由P97启动子转录而来，受细胞转录因子和病毒E2基因产物的调控。体内和体外实验表明，HPV-16E2蛋白具有与BPV-1E2蛋白相同水平的反式激活异源启动子的能力。5.成功研制了百伦激光热疗机，并将其应用于鼻变态反应和上颌癌患者，取得了较好的临床效果。

1.An in vitro culture system in which human respiratory epithelial cells can grow and differentiate into both ciliated and secretory cells was established. Using these floating cultured cells, the studies on the regulation of cell differentiation due to some cytokines as well as the ciliary metachronism due to exogenous ATP,endotoxin and macrolide antibiotics were performed.2.It was revealed that IL-1beta is predominant cytokine in sinus fluid of chronic sinusitis and is produced by polymorphonuclear neurophils, induced the expression of ICAM-1 and ELAM-1 on endothelial cells from the results of both immunohistchemical and biochemical studies, in addition to IL-1beta mRNA detection by in situ hybridization.3.Lectin histochemisty showed that both increased staining of glycoproteins such as fucose and sialic acid and diferent composition of these terminal carbohydrates in addition to dominantly reduced intensity of saccharide chain of mannose were observed in sinusitis mucosa. There were increased sttaining of PNA in well differntiated type and it of SNA in anaplastic type of laryngeal squamous cell carcinoma.4.A predominance of HPV-11 was detected by PCR among recurrent airway papillomatosis (RAP). The presence of antibodies against the recombinant E6 and L2 proteins of HPV-11 subcloned were demonstrated serologically in RAP patients. The E6 and E7 genes of HPV-16 were transcribed from the P97 promotor and were regulated by cellular transcriptional factors and viral E2 gene product. It was showed that HPV-16 E2 protein had the ability to trans-activate the heterogenous promotor at the same level of BPV-1 E2 protein in the in vivo and in vitro experiments.5.Baloon laserthermia equippment was deviced successfully and was applied into the patients with nasal allergy and maxillary cancers showing the fairly good clinical results.