Development of Slow Releasing Anticancer Drug Based with Absorbable Biomaterial Chit*

我们研究了顺铂和甲壳素复合后的铂金的缓释性能和抗癌作用。本研究对其缓释性能进行了较为详细的研究，并对其肾脏并发症及对实体瘤的疗效进行了小鼠和兔的实验研究。将铂(棉型)植入小鼠腹壁后，测定不同脏器中铂的浓度。在植入铂的腹肌中，铂的浓度一直维持到8周，在植入后4周达到高峰。与此同时，铂的血清浓度保持在较低水平。在肾脏中，铂的浓度与腹肌中的浓度相似，但在血清学或病史上没有发现肾功能障碍。与对照组相比，在实体瘤周围植入Plachtin可提高存活率，抑制肿瘤重量的增加。…从这些发现中可以看出，作为一种局部应用的缓释抗癌药物，Plachtin似乎是有效的。我们还研究了用普拉丁进行动脉内化疗栓塞术。一克普拉丁含有300 mg顺铂，并将其修饰成颗粒(直径约50微米)。将VX2肿瘤接种于兔的后肢。当VX2肿瘤生长2 cm时，股动脉注射铂。这项研究分四组进行：普拉丁、顺铂、甲壳素和对照组。普拉丁组肿瘤生长率明显低于其他组(p&lt；0.05)。仅普拉丁组肿瘤消退。肿瘤铂水平高于血清和肾铂水平。普拉丁组的血尿素氮和血肌氨酸水平均在正常范围。以上结果表明，铂颗粒在肿瘤周围缓慢释放顺铂，并通过顺铂和栓塞剂的相加作用抑制肿瘤生长。普拉丁的肾毒性尚未被认识到。铂可作为化疗栓塞剂用于癌症患者的治疗。较少

We studied the slow releasing property and anticancer, effect of Plachitin, which is reconstituted by combination of CDDP and chitin. This study deals in more detail with the slow releasing property, and the renal complications and the effectiveness for solid tumor were examined in mice and rabbits.1. After implantation of Plachitin (cotton type) in the abdominal wall of mice, the platinum concentration in the different organs was measured. In the abudominal muscle around the implanted Plachitin, a high concentration of platinum was maintained until 8 weeks and the the peak was 4 weeks after implantation. At the same time, the serum concentration of platinum remained low. In the kidney, the platinum concentration resembled the levels in the abdominal muscle, but no renal dysfunction was found serologically or historogically.2. When Plachitin was implanted around the solid tumor, the survival rates were improved and the gain in tumor weight was suppressed as compared with the controls. … More From these findings, Plachitin seemed to be effective as a slow releasing anticancer drug for topical application.3. We studied about intraarterial chemoembolization therapy by use of Plachitin too. One gram of Plachitin contained 300mg CDDP and the form of Plachitin was modified into particles (about 50um in diameter). VX2 tumor was inoculated in hind limb of rabbits. When VX2 tumor was grown 2cm in diameter, Plachitin was injected in the femoral artery. The study was carried out in four groups : Plachitin, CDDP, chitin, and control groups. The tumor growth ratio was significantly lower in Plachitin group compared with other groups (p<0.05). Tumor regression was noticed in Plachitin group only. The tumor Platinum (Pt) level was higher than serum and kidney Pt.level. Blood urea nitrogen and Ceratinine level were normal range in Plachitin group. From the above results it can be concluded that Plachitin particles released CDDP slowly around the tumor and the tumor growth was suppressed by the additive effect of CDDP and embolization. Renal toxicity of Plachitin was not recognized. Plachitin could be considered as an useful agent for chemoembolization therapy for the cancer patients. Less