Analysis of immunosupprative meclauism of Lipocortiut in tuwcr-beurig hestsits expression and action in wan

在这项研究中，我们探讨了脂皮质素-1（LC1）在人体组织中的表达及其在抗癌自卫机制中的作用。研究结果如下：1)通过手术标本免疫组化研究，证实肿瘤基质中炎性细胞（主要是巨噬细胞）胞浆中存在LC1。2)通过FACS分析，来自癌症患者和健康志愿者的外周血单核细胞（PBMC）中LeuM3和LC1阳性细胞的比例分别为20%和5%。3)通过western blot分析，在胃癌患者的恶性腹水中检测到分子为35或37 KDa的LC1蛋白。4)在PBMC培养物中加入不同浓度的重组小鼠LC1(γ -LC1)，评估其对健康供体有丝分裂反应（Con A）的影响。有LC1（0.2、2.0、20mug/ml）时的有丝分裂反应明显低于无LC1组（10%、90%、99%）。此外，用肿瘤患者脾细胞的恶性腹水或培养上清代替lc1，对有丝分裂反应的抑制作用更明显。6)癌症患者的PBMC有丝分裂反应比健康供者下降30%。利用尼龙棉柱制备癌症患者的PBMC，以去除贴壁细胞，使反应逆转到正常水平。在癌症患者中，由于LC1在有丝分裂反应中具有特殊的抑制活性，LC1的产生是明显的，它似乎与明天宿主的免疫抑制有关。我们也认为LC1在生鼠体内的表达与肿瘤的进展密切相关，因此我们正试图通过定量检测人血清中的LC1蛋白来阐明LC1的表达与癌症患者肿瘤的进展之间的关系。少

In this study, we have explored the expression of Lipocortin-1(LC1)in the human tissues and its role in self-defense mechanism against cancers. The results are as follows ; 1)By immunohistochemical study using operative specimens, LC1 was proved in the cytoplasma of the inflammatory cells(mainly Macrophages)in the matrix of the tumor. 2)By FACS analysis, the rate of both LeuM3 and LC1 positive cells in peripheral blood mononuclear cells(PBMC)derived from cancer patients and healthy volunteers were 20% and 5% respectively. 3) By Westem blot analysis, LC1 protein whose molecule was 35 or 37 KDa was detected in the malignant ascites of gastric carcinoma patients. 4)Various concentrations of recombinant mouse LC1(gamma-LC1)were added to the cultures of PBMC to evaluate the effects on mitogenic responses(Con A)in healthy donors. The mitogenic responses in the presense of LC1(0.2, 2.0, 20mug/ml)were severely lower(10%, 90%, 99%)as compared to those without LC1. Furthermore, the similar inhib … More itory effects on mitogenic responses were obtained when malignant ascites or culture supernatents of spleen cells from cancer patients were added to the cultures instead of LC1.5)These inhibitory effects on the mitogenic responses were reversed by the addition of anti-LC1 serum. 6)Mitogenic responses of PBMC in cancer patients showed a decrease of 30% over healthy donors. Preparation of PBMC from cancer patients using nylonwool column in order to remove adherent cells reversed the response to normal level.In cancer patients, LC1 is produced distinctly and it seemed to be concerned in immunosuppression in tomor-bearing hosts because of the peculiar suppressive activities of LC1 in mitogenic responses. It is also suggested that LC1 is expressed strongly in accordance with progress of tumor in tomor-bearing mice, therefore we are now trying to clarify the relationship between expression of LC1 and progress of tumors in cancer patients with an effort to detect LC1 protein quantatively in human serum. Less