Studies on movement of sinusoidal cells in the liver

用相衬显微镜观察从大鼠肝脏分离的肝星状细胞的形态。培养的星状细胞不断改变其形态。在塑料上播种后，星状细胞在细胞核周围延伸出薄的扇形膜状突起。当这些膜状突起进一步扩大时，突起在外观上变为树突状。两天后，细胞逐渐由树突状变为膜状。当在星状细胞中加入KCCM后2天，膜状突起明显转变为树突状突起。为了了解刺激KCCM树突形成的潜在介质，在原代培养中加入PGE_2。在加入PGE_2后，星状细胞形态也发生了类似的变化（Wake， K.et al.1993）。通过计算机辅助重建高尔基染色切片、透射电镜和扫描电镜，观察肝窦壁的三维结构和椎间盘间隙。从解剖学的角度来看，星状细胞的收缩和松弛或内皮细胞承受的一定压力可以加速血流与椎间盘间隙通过内皮开孔的流体交换（Wake， K.1995）。为了探讨淋巴细胞和单核细胞与内皮细胞的粘附模式，我们在配备摄像机的显微镜下观察了活体鼻窦炎。注射四氯化碳后，内皮细胞黏附大量单核细胞，部分单核细胞呈变形体运动。肉芽肿内部只有机械壁，但有动态细胞复合体。

Morphology of the hepatic stellate cells isolated from the rat liver was observed phase-contrast microscorpy with or without Video-recording. Cultured stellate cells change their forms continuously. After seeding on plastic, stellate cells extend thin fan-shaped membranous processes around the nucleus. As these membranous processes expand further, the processes change to dendritic in appearance. Two days after seeding the cells change their shapes gradually from dendritic to membranous. When kupffer cell-conditioned medium (KCCM) is added to stellate cells two days after seeding, the membranous processes change dramatically to dendritic. To know the potential mediators stimulating the formation of dendritic processes in KCCM,PGE_2 was added to the primary culture. Similar changes in stellate cell morphology occured rapidly after adding PGE_2 (Wake, K.et al.1993).Three-dimensional structure of the sinusoidal wall and the space of Disse in the liver were examined by the computer-assisted reconstruction of serial sections of the Golgi stained preparations, transmission and scanning electron microscopy. From the anatomical point of view, contraction and relaxation of the stellate cells or certain pressure loaded upon the endothelial cells can accelerate fluid-exchange between the blood stream and the space of Disse through the endothelial fenestrations (Wake, K.1995).In order to explore the mode of adhesion of lymphocytes and monocytes to endothelial cells we observed the sinusoid in vivo under a microscope equipped with a Video-camera. After injection of carbon tetrachloride, numerous mononuclear cells sticked to the endothelial cells and some of them showed ameboid movement. Within the granuloma formations only a mechanical wall, but a dynamic cell-complex.