Immunological and nutritional effects growth factor in small bowel transplantation

小动物，如大鼠，因其易于操作和应变显示清楚而常被用作小肠移植的实验模型。然而，我们已经花费了大量的时间来建立大鼠移植模型的外科手术程序。较短的移植肠段有利于减少排斥反应，但不利于营养物质的吸收。我们报道了表皮生长因子(EGF)可增强大鼠小肠移植后的肠道适应。已有报道称EGF可增强大范围肠切除后受损肠道的适应。为探讨表皮生长因子(EGF)对大鼠小肠移植后肠功能恢复的影响。受体Lewis大鼠接受了小肠远端80%的切除，取而代之的是20厘米的同种异体移植。移植后给予EGF(30mug/kg/d)或其载体，连续3d。7d后，分离移植物进行形态观察，并进行葡萄糖和水分吸收及葡萄糖转运蛋白1(SGLT1)表达的分析。EGF治疗组大鼠粘膜绒毛高度、隐窝细胞增殖、葡萄糖和水分吸收以及SGLT1蛋白表达均显著增加。两组在体重变化和隐窝深度方面无显著差异。这些结果表明，EGF增强了大鼠移植肠的结构和功能适应。在短肠综合征患者的肠道移植后，EGF可能是有用的。

Small animals such as rats are often used for the experiment model ofsmall bowel transplantation since they are easy handling and their strain is shown clearly. However, we have spent lots of times to establish the surgical procedure of transplant model in rats. A short segment of intestinal graft is favorable for reducing rejection, but less favorable for absorption of nutrients. We report that epidermal growth factor (EGF) augments the intestinal adaptation after small bowel transplanatation in rats.EGF has been reported to enhance adaptation in damaged intestines following massive intestinal resection. Studies were performed to determine whether EGF influences the recovery of intestinal function after small bowel transplanation in rats. Recipient Lewis rats underwent resection of the distal 80% of the small bowel, which was replaced with a 20 cm isograft. EGF (30mug/kg/day) or its vehicle was infused intraperitoneally for 3days after transplantation. After 7days, the graft was isolated for morphologic studies and was used for analysis of glucose and water absorption and the expression of sodium glucose contransporter1 (SGLT1). These were used as indicators of functional adaptation.The EGF-treated group exhibited significantly incteased mucosal villous height crypt cell proliferation glucose and water absorption, and expression of SGLT1 protein. No significant differences were found in body weight change or crypt depth between the two groups. These results demonstrate that EGF augments structural and functional adaptation of intestinal grafts in rats. EGF may be useful after intestinal transplanatation in patients with short bowel syndrome.