A molecular hub regulating root stem cell replenishment in Arabidopsis

植物根系发育中最重要的问题之一是根系中干细胞稳态是如何响应许多不同的外部和内部信号而受到调节的。当干细胞由于分化或不同的压力（如遗传毒性）而丢失时，根的干细胞库必须保持并不时地通过静止中心（QC）细胞的协调分裂来补充。许多不同的因素，如植物激素和转录调节因子，已知在调节QC分裂和干细胞补充中发挥重要作用。然而，许多不同因素之间的相互关系在很大程度上是未知的。在这个拟议的项目中，我们的目标是仔细检查一些局部和一些更一般的干细胞补充调节的新出现的联系。此外，转录调控因子在不同亚核区域的定位将分析其在干细胞维持和分化的必要微调平衡中的功能。总之，在这个项目中，我们的目标是揭示参与根干细胞补充的不同转录调控因子的高度交错的调控和相互作用。

One of the most important questions in plant root development is how stem cell homeostasis is regulated in the root in response to the many different external and internal cues. The root’s stem cell pool has to be maintained and from time to time replenished by coordinated divisions of the quiescent center (QC) cells when stem cells are lost due to differentiation or different stresses, e.g. genotoxicity. A multitude of different factors such as phytohormones and transcriptional regulators are known to play important roles in regulating QC divisions, and therefore stem cell replenishment. Still, most of the interconnections of the many different factors are largely unknown. In this proposed project, we aim to scrutinize newly emerging links of some local and some more general regulators of stem cell replenishment. Furthermore, the localization of transcriptional regulators in differential sub-nuclear domains will be analyzed for their function in the necessary fine-tuned balance of stem cell maintenance and differentiation. Taken together, in this project we aim to uncover the highly interleaved regulation and interaction of different transcriptional regulators involved in root stem cell replenishment.