Maintenance and Function of the Barley Root Apical Meristem

大麦根顶端分生组织的维护和功能

• 批准号: 467850977
• 负责人: Professorin Dr. Yvonne Stahl
• 金额: --
• 依托单位: Institut für Molekulare Biowissenschaften
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/467850977?language=en
• 关键词: Maintenance; Function; Barley; Root; Apical

The stem cell niche (SCN) in the root apical meristem (RAM) provides the pool of stem cells necessary for the establishment and maintenance of the whole root system of a plant. Until now, most of the research on RAMs has been carried out in the eudicot model plant Arabidopsis thaliana. Here, it was shown that in addition to the phytohormones auxin and cytokinin, some of the key transcription factors (TFs) e.g., WUSCHEL RELATED HOMEOBOX 5, PLETHORA 1-4 and SHORTROOT and SCARECROW play important roles in RAM maintenance. In an intricate interplay these TFs regulate quiescent center positioning and divisions, which is crucial to a functional SCN and therefore also for a functional RAM. In monocot cereals like barley very little is known about the molecular factors controlling the necessary tight but also flexible regulation from stem cell fate to differentiation. Primary experiments suggest a more elaborate molecular regulation of the observed bigger stem cell pool in the SCN of the barley RAM generating also additional cell layers. Moreover, some predicted homologous molecular regulators are not expressed or in different cells indicating different functions in the barley RAM compared to Arabidopsis. To understand how the RAM of cereals is organized, maintained and how it functions, we aim to i) identify until now unknown molecular factors regulating the barley RAM as a cereal model and, in addition, ii) scrutinize the homologs of known molecular factors from Arabidopsis for their functional relevance in barley RAM maintenance.

根顶端分生组织中的干细胞龛（SCN）为植物整个根系的建立和维持提供了必要的干细胞库。到目前为止，大多数关于RAM的研究都是在真双子叶植物模式植物拟南芥中进行的。在此，研究表明，除了植物激素生长素和细胞分裂素，一些关键的转录因子（TF），例如，WUSCHEL相关的HOMEOBOX 5，PLETHORA 1-4和SHORTROOT和SCARECROW在RAM维护中起着重要作用。在一个复杂的相互作用，这些TF调节静态中心的定位和分工，这是至关重要的功能SCN，因此也为一个功能的RAM。在单子叶植物谷物如大麦中，对控制从干细胞命运到分化的必要的紧密但也灵活的调节的分子因子知之甚少。初步实验表明，在大麦RAM的SCN中观察到的更大的干细胞池的更精细的分子调控也产生额外的细胞层。此外，一些预测的同源分子调节器不表达或在不同的细胞中，表明与拟南芥相比，大麦RAM中的不同功能。为了了解谷物的RAM是如何组织的，维持的，以及它是如何发挥作用的，我们的目标是i）鉴定到现在为止未知的调节大麦RAM的分子因子作为谷物模型，并且，此外，ii）仔细检查来自拟南芥的已知分子因子的同源物，以了解它们在大麦RAM维持中的功能相关性。