Construction of physical map chicken W chromosome

用雌性鸡基因组DNA的限制性内切酶Sau3A I和cosmid载体super Cos1构建鸡基因组文库，用鸡W染色体特异性重复序列pUGD0601 （Xho I家族0.7kb单位）和pUGD1201 （EcoR I家族1.2kb单位）进行分析。鸡W染色体特异性DNA文库分别为900克隆Xho I家族和600克隆EcoR I家族。斑点杂交分析结果显示，85%的克隆具有W染色体特异性序列，表明该文库覆盖了鸡W染色体基因组总量的72%。利用southern blot杂交技术对超级Cos 1的短臂和长臂进行分析，构建了每个克隆的限制性内切酶切割图谱，并利用DNA指纹图谱对每个克隆进行分析。这些结果表明，插入物的平均大小约为40kb，这些文库相当于整个W染色体基因组的2.1%。

Chicken genomic library which was constructed with both of restriction enzyme Sau3A I digest of female chicken genomic DNA and cosmid vector super Cos1 were analyzed with two chicken W chromosome specific reiterated sequences pUGD0601 (a 0.7kb unit of the Xho I family) and pUGD1201 (a 1.2kb unit of the EcoR I family). It was detected the chicken W chromosome specific DNA library with 900 clone of Xho I family and 600 clone EcoR I family, respectively. In the results of dot blot hybridization analysis, the 85% clone had W chromosome specific sequences, and it was suggested that this library covered 72% of the amount of chicken W chromosomal genome. The restriction enzyme cleavage map of each clone were constructed by southern blot hybridization analysis among the cosmid clone and short and long arm of the super Cos 1, and analyzed each clone by using DNA finger printing. These results suggested that the mean of the size of insert was about 40kb, and these library were equivalent to 2.1% of whole W chromosome genome.