Studies on the Valuation of Satsuma Native Fowl as Animal Genetic Resources.

萨摩土禽动物遗传资源价值研究。

• 批准号: 01560295
• 负责人: HASHIGUCHI Tsutomu
• 金额: $ 1.02万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560295/
• 关键词: Satsuma native fowl; Animal genetic resources; Muscle protein turnover rate; Fractional rates of synthesis and degradation; Genetic parameter; Feed efficiency; Genetic variability; RFLP; DNA切断片長多型; RFLP; 矮性系薩摩鶏

1) Age at first laying, egg weight, egg production to 500 days of age and body weight at 300 days of age in Satsuma native fowls as animal genetic resources over 2 generations. Heritabilities of the above mentioned traits and genetic and phenotypic correlations among them were estimated.2) Fractional rates of muscle protein synthesis and degradation were determined by measuring the output of N^1-methylhistidine in the excreta in the chicken. The fractional rates of synthesis and degradation at 4 weeks of age in the Satsuma native fowl were relatively high compared with these in the other stocks.3) The proportion of the polymorphic loci (Ppoly), the expected average heterozygosity per individual (H), and the effective number of alleles per locus (Ne) were assayed to estimate the genetic variabilities in Satsuma native fowl. That is, Ppoly=0.22, H=0.08 and Ne=1.1.4) A feeder for measuring chicken feed intake was designed, and the genetic parameters of feed efficiency in Satsuma native fowl.5) Chicken genomic DNAs were prepared from 13 lines of the chicken including Satsuma native fowl and digested with 6 restriction enzymes RFLP was applied to the digested DNA using random clone as a probe. Hybridization banding patterns of Satsuma native fowl were identical among the 13 lines.

1)以2代以上萨摩土禽为动物遗传资源，研究其初产蛋龄、产蛋量、500 ~ 500日龄产蛋量和300日龄体重。估计了上述性状的遗传力和遗传表型相关性。2)通过测定鸡排泄物中N^1-甲基组氨酸的排泄量，确定肌肉蛋白质合成和降解的分数率。在4周龄时，萨摩原禽的合成和降解分数率相对于其他种群较高。3)通过分析多态位点比例（poly）、个体期望平均杂合度(H)和每个位点有效等位基因数（Ne）来估计萨摩原鸡的遗传变异。即poly=0.22， H=0.08, Ne=1.1.4)设计了一种测定鸡采食量的饲养器，并测定了萨摩原禽饲料效率的遗传参数。5)采用随机克隆为探针，用6种限制性内切酶对鸡基因组DNA进行RFLP分析。13个品系间杂交带型基本一致。

项目成果

Yamashita H., Y. Maeda, S. Okamato and T. Hashiguguchi: "On the DNA Restriction Fragment Length Polymorphism (RFLP) in Chickens" Jpn. Poult. Sci.27(November). 1-8 (1990)

Yamashita H.、Y. Maeda、S. Okamato 和 T. Hashiguguchi：“论鸡的 DNA 限制性片段长度多态性 (RFLP)”Jpn。

Maeda Y., S. Okamoto, K. Okano, T. Tomita and T. Hashiguchi: "The comparison of muscle protein turnover rate among the egg type, meat type and Japanese native chicken stocks." Jpn. J. Zootech. Sci. 61. 701-706 (1990)

Maeda Y.、S. Okamoto、K. Okano、T. Tomita 和 T. Hashiguchi：“鸡蛋类、肉类类和日本本土鸡汤之间肌肉蛋白周转率的比较。”

Yoshizane MAEDA: "The Comparison of Muscle Protein Turnover Rate among the Egg Type,Meat Type and Japanese Native Chicken Stocks" Japanese Journal of Zootechnical Science. 61. 701-706 (1990)

前田义真：“蛋类、肉类和日本本土鸡种的肌肉蛋白周转率比较”《日本动物技术科学杂志》。

橋口勉: "薩摩鶏の飼料効率に関する遺伝的パラメ-タ-について" 日本家禽学会誌.

Tsutomu Hashiguchi：“与萨摩鸡饲料效率相关的遗传参数”，日本家禽学会杂志。

山下 秀次: "ニワトリにおけるDNA切断片長多型(RFLP)について" 日本家禽学会誌秋季大会号. 27. I-8 (1990)

Hidetsugu Yamashita：“关于鸡的 DNA 片段长度多态性 (RFLP)”，日本家禽协会秋季会议第 27 期。I-8 (1990)。