Glucose transport and glycolytic enzyme activities in erythrocytes and various tissues of animals

本文测定了狗、猪、猫、马、牛、羊的红细胞D-葡萄糖转运蛋白(D-GT)、糖酵解酶己糖激酶(HK)和丙酮酸激酶(PK)的活性。狗的红细胞D-GT、HK和PK活性最高，显著高于食草动物的红细胞。猫红细胞D-GT和HK活性显著低于狗。不同种马红细胞D-GT活性的差异遵循HK活性的差异，而不是PK活性和血糖浓度的差异。测定了2岁纯种马在连续训练运动(赛马)下的D-GT转运和胞浆酶活性，并与不同年龄的未受过训练的马(父马、母马和未受过训练的2岁PLD纯种马)进行了比较。葡萄糖转运和糖酵解酶…的活性赛马红细胞中的HK和PK_2含量比未经训练的马高2~3.5倍。受训和未受训的马匹之间的血糖、甘油三酯或免疫活性胰岛素浓度没有显著差异。红细胞葡萄糖转运和糖酵解酶活性的增加被认为反映了训练后赛马代谢活性的增加。对正常乳腺和乳腺肿瘤的D-GT转运和胞浆酶、HK、PK和乳酸脱氢酶(LDH)的活性进行了测定。肿瘤组织中D-GT活性高2倍，HK和PK活性高3-4倍。这似乎是由于肿瘤细胞对葡萄糖的摄取和利用增加。兔抗GLUT1抗体免疫印迹检测到狗、猫、猪、马、牛、羊红细胞膜上存在GLUT1(Hep G2型)。较少

The activities of D-glucose transtport (D-GT) and the glycolytic enzyme hexokinase (HK) and pyruvate kinase (PK) were measured in the erythrocytes of dogs, pigs cats, horses, cattle and sheep. The erythrocytes of the dog had the highest activities of D-GT,HK and PK,singificantly higher than the activities in the erythrocytes of the herbivores. The activities of D-GT and HK in erythrocytes of the cat were significantly lower than in those of the dog. The differences between the activities of D-GT in the erythrocytes of the different species followed the differences in activities of HK but not those in the activities of PK or in the blood glucose concentration.D-GT transport and cytosolic enzyme activities were measured in erythrocytes of 2-year-old thoroughbreds under continuous training exercise (race horses) and compared with those of untrained horses in various ages (sires, mares and untrained 2-year-pld thoroughbreds). The activities of the glucose transport and glycolytic enzymes, … More HK and PK,in the erythrocytes of the race horses were elevated to 2-3.5 times above those of untrained horses. There were no significant differences in plasma glucose, triglyceride or immunoreactive insulin concentrations between the trained and untrained horses. The increases in glucose transport and glycolytic enzyme activities in their erythrocytes are considered to reflect an increased metabolic activity in the race horses resulting from the traning exercises.D-GT transport and cytosolic enzymes, HK,PK and lactate dehydrogenase (LDH), activities were measured in the normal mammary gland and the mammary gland tumor of the dog. D-GT activitiy was 2 times higher and, HK and PK acitivities were 3-4 times higher in the tumor tissues. It seems to be increased intake and utilization of glucose in the tumor cells. LDH activity was 2 times higher in the tumor tissues and LDH isozyme V was predominated in malignant mammary tumors.GLUT1 (Hep G2 type) was observed in the erythrocytes membrane of dogs, cats, pigs, horses, cattle, sheep by western blotting using GLUT1 antibody, which was developed in the rabbits. Less