Expression of human IL-2 receptor beta and gamma chains in mucosal immue lymphatic tissue and the immunopathological meaning.

阐明促进靶细胞或肿瘤细胞分裂或增殖的生长因子的信号转导是一个重要而迫切的需要。1）IL-2 R β的表达定位于细胞膜表面; 2）免疫胶体金法检测IL-2 R β的表达量约为IL-2 R α的1/20; 3）IL-2 R β作用后5 min，IL-2 R β的内化达到高峰; 4）作用后30 min，IL-2 R β定位于核间隙。这些结果证实了IL-2 β参与细胞增殖的生化原理，并且我校Sugamura等制备了抗IL-2 R γ。现在，我们检查了MT-2、MT-1和ILT-Mat细胞系中抗IL-2 R γ的定位。本实验在人胃肠道粘膜、肿瘤、结肠癌、克罗恩病和溃疡性结肠炎的结肠组织中检测到抗IL-2 R β抗体。

It is an important urgentnecessity to clarity the signaltransduction of growth factors which make division of or proliferate target cells or tumorcells.We elarified in the signal transduction of IL-2 receptor beta chans that ;1) The expression of IL-2Rbeta was located in surface membrane, 2) The number of IL-2Rbeta was 1/20 of IL-2Ralpha by immuno-gold technique, 3) 5 minutes after the treatment of IL-2Rbeta, the internalization of IL-2Rbeta was a peak, and 4) 30 minutes after the treatment, IL-2Rbeta was localized at phinuclear space. These results confirm biochemical those that IL-2beta participates in the prdiferative of cells.Furtheremore, Sugamura et al. of our university prepared anti-IL-2Rgamma. Now, we examined the localization of anti-IL-2Rgamma in cell lines of MT-2, MT-1, and ILT-Mat. We detected the same localization of anti-IL-2Rgamma in those cells as served in IL-2Rbeta.We are in the way of detecting the anti-IL-2Rbeta localization in human gastrointestinal mucosa, their tumors, colen cancers, and colons of Cromn's disease and Uleerative colitis.