Human papillomavirus type-specific cytopathogenic effect

(1)我们发现两种新的胞浆内包涵体（ICBs）与不同类型的人乳头瘤病毒的不同临床特征和DNA存在相关。在所有已知的颗粒状ICBs病变中检测到HPV 1 DNA序列。具有丝状ICBs的点状角化病变与新近从这种病变中克隆的HPV 63相关。具有均匀ICBs的色素疣含有一种相关的HPV，即HPV 4， HPV 60或新型HPV，HPV 65。基于这些关联，提出了包涵疣的分类。(2)虽然手掌和脚底的表皮样囊肿长期以来被认为是由于穿透性损伤而植入表皮碎片后形成的，但其病理机制仍然存在争议。本文对119例掌足底表皮样囊肿进行了临床、组织学、免疫组织化学和分子生物学研究，以探讨内分泌管和人乳头瘤病毒（HPV）在这种疾病发病机制中的作用。在73例（61%）中发现癌胚抗原表达的导管结构。36例（30%）检测到乳头瘤病毒常见抗原，表现出三种不同组织学特征中的一种或多种，即胞浆内包涵体（ICBs）、空泡结构和囊肿腔角化不全。根据我们的研究结果，我们认为掌足底的表皮样囊肿可能是由内分泌管发展而来的，HPV可能在其发病机制中起作用。

(1) We revealed that two new types of intracytoplasmic inclusion bodies (ICBs) were associated with distinct clinical features, and the presence of DNA of distinct types of human papillomaviruses. HPV 1 DNA sequences were detected in all the lesions with well-known granular ICBs. Punctate keratotic lesions with filamentous ICBs were associated with HPV 63, which was newly cloned from such a lesion. Pigmented warts with homogeneous ICBs contained one of the related HPVs, i.e., HPV 4, HPV 60 or a novel type of HPV,HPV 65. Based on these associations, a classification of inclusion warts is proposed.(2) Although epidermoid cysts of the palms and soles have long been assumed to develop following implantation of an epidermal fragments as a result of a penetrating injury, the pathomechanism is still controversial. Clinical, histological, immunohistochemical and molesular biological studies were performed on 119 epidermoid cysts of palmoplantar location, inorder to examine the role of the eccrine ducts, and human papillomavirus (HPV) , in the pathogenesis of this disorder.Either ductal structures of carcinoembryonic antigen expression were noted in a total of 73 cases (61%). Papillomavirus common antigens were detected in 36 cases (30%) showing one or more of the three distinct histological features, i.e., intracytoplasmic inclusion bodies (ICBs) , vacuolar structres and parakeratosis in the cyst cavity. On the basis of our results, we propose that epidermoid cysts in the palmoplantar regions may develop from eccrine ducts, and that HPV may play a role in their pathogenesis.